Biological control bacillus subtilis for crop bacterial wilt

A technology of Bacillus subtilis and bacterial wilt, which is applied in the direction of plant growth regulators, bacteria, biocides, etc., can solve the problems that the physiological and biochemical characteristics of biological control bacteria and disease prevention mechanisms have not been studied in depth, and achieve dark green leaf color and strong growth. Good, the effect of leaf color hypertrophy

Inactive Publication Date: 2006-12-27
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] There have been reports on the biological control of tobacco bacterial wilt in China, but most of them are limited to the screening of bio-control bacteria and the stage of pot plant experiments, and many aspect

Method used

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  • Biological control bacillus subtilis for crop bacterial wilt
  • Biological control bacillus subtilis for crop bacterial wilt
  • Biological control bacillus subtilis for crop bacterial wilt

Examples

Experimental program
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Effect test

Embodiment 1

[0064] 1.1 Screening of antagonistic bacteria SH7 for the control of tobacco bacterial wilt, greenhouse pot plant disease control experiment and growth promotion effect

[0065] Evenly add 100 μl of Ralstonia solanacearum (Ralstonia solanacearum) bacterial suspension with a concentration of 1×107 cfu / ml into a sterilized petri dish, pour into 20 ml of molten NA medium cooled to about 50° C. and mix well. After the culture medium is completely solidified, use an inoculation loop to inoculate the antagonistic bacteria to be tested, spot 8-10 bacterial strains to be tested in each dish, and culture them in a 30°C incubator for 48 hours to detect the presence and size of the inhibition zone. The strains with antagonistic effect in the initial test were streaked and purified, and the test was repeated. The basic method was the same as above. Three strains were spotted on each plate, and each was repeated three times. From the antibacterial effect of the plate, the indoor plate of t...

Embodiment 2

[0091] Example 2 SH7 antagonistic protein and its properties

[0092] Extraction of crude protein and detection of its activity: inoculate the activated SH7 strain in NB medium, culture at a constant temperature of 30°C, shake at 150rpm for 48 hours, centrifuge at 4°C for 10 minutes, remove bacteria, and make extracellular metabolites for fermentation Serum. Slowly add a certain amount of (NH4)2SO4 to the fermentation broth to reach a saturation of 70%, stir evenly, and place it at 4°C overnight.

[0093] Centrifuge the salting-out solution at low temperature for 20 minutes (12000r / min), pour off the supernatant, suspend the precipitated substance with 1 / 15 volume of PBS buffer solution (pH7.6), and put the suspension into the pretreated dialysis bag (Cut-off molecular weight is 5kD), the two ends of the dialysis bag are clamped with dialysis clips, placed in 100 times the volume of PBS buffer (pH7.6), dialyzed on a magnetic stirrer at 4°C for 24 hours, and then dialyzed for ...

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Abstract

The invention provides a crop Pythium biological control bacillus subtilis strain and belongs to the biotechnological sphere. The invention relates to a crop Pythium bacillus subtilis strain and its biological crop preserved number is CGMCC1732. The said strain can reduce the growth of Ralstonia solanacearum and the biological control experiment indicates that it can prevent and cure the Granville wilt. Simultaneously the invention also provides an antagonistic protein blend obtained from the said strain and the blend has higher application value in crop Pythium biological control.

Description

technical field [0001] The invention belongs to the field of biotechnology. Further, the present invention relates to a strain of Bacillus subtilis for the biocontrol of bacterial wilt. Furthermore, the present invention relates to the application of Bacillus subtilis strain SH7 to control tobacco bacterial wilt. Background technique [0002] Tobacco bacterial wilt is a typical vascular disease caused by Ralstonia solanacearum. The most prominent symptom is withering, and the withering speed is very fast. Once the disease occurs, the whole plant can die , is a devastating disease on tobacco. At present, it generally occurs in the Yangtze River Basin and the south of the tobacco area in my country; the incidence rate of dry land tobacco fields in frequently-affected areas is 30%-50%, especially the incidence rate of dry land continuous cropping tobacco fields is more than 50%; especially South China tobacco The area is severely damaged, and outbreaks occur in individual yea...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02C07K14/32C12R1/125
Inventor 赵廷昌王静孔凡玉
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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