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Extraction of activated micromolecular sheept germine from sheep embryo and its use

A small molecule, sheep placenta technology, applied in the field of bioengineering, can solve the problems of high requirements and high risks of new products

Active Publication Date: 2007-01-17
YANGZHOU YANGDA LIANHUAN PHARMA GENE ENG
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the risk of injection is relatively high, and the requirements for new products are also high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] 1. Prepare activated small molecule sheep placenta according to the following steps:

[0028] 1. Take fresh goat embryos aged 40-90 days, remove the intestines and intestines, and wash them.

[0029] 2. Cut or chop.

[0030] 3. Add distilled water and stir to make a homogenate.

[0031] 4. Freeze and thaw the homogenate three times at -20°C at -20°C, adjust the pH value to 3.0-3.5, filter, and then adjust the pH value of the filtrate to 7.0.

[0032] 5. Heat the filtrate to 70±1°C, keep it warm for 30±2 minutes, centrifuge for 30±2 minutes under the condition of centrifugal force of 8000g, and obtain filtrate A and sediment A respectively;

[0033] 6. Ultrafiltration of the filtrate A, the ultrafiltration conditions are: pH 7.0, flow rate 20-40ml / min. After ultrafiltration, the supernatant should be transparent and light yellow. Take the filtrate B and precipitate B.

[0034] 7. Combine the precipitates A and B, adjust the pH to 2.0, add pepsin, and incubate at 37±2...

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PUM

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Abstract

The extraction of active small molecular placentin from lamb embryo and its purpose that belongs to the bioengineering field includes the following steps: removing stomach and intestine from fresh lamb embryo of 40-90 days and cutting up, homogenizing with distilled water, frost thawing repeatedly at -20DEG C, filtrating, heating the filtrate and obtaining Filtrate A and Sediment A; ultra-filtrating Filtrate A and obtaining the transparent primrose yellow Filtrate B and Sediment B; mingling Sediment A and Sediment B, modifying pH to 2.0, adding pepsin, then modifying pH to 7.0, incubating at 100 +-2DEG C and preserving Filtrate C; active small molecular placentin is obtained by mingling Filtrate B and C that can be used in the preparation of skin care product. The placentin prepared in this invention has the molecular weight of 3000-6000 and contains mainly cytokines and small peptides, has high activity, low side-effect such as anaphylactic response, and increased skin absorption ratio.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and the technology is applied to the preparation and production of activated small-molecule sheep placenta, which can be used as a raw material to produce cosmetic products for external use. Background technique [0002] Sheep placenta extracts living cells MIENS from sheep placenta through biotechnology, and 100% maintains the active substance of the embryo. Sheep placenta is rich in lecithin, cephalin (brain cell nutrient), lipopolysaccharide, multivitamins and essential constants, trace elements, various immunoglobulins, various antibodies, interferon, thymosin (to maintain cell Immune function, anti-aging), active peptides, epidermal growth factor (maintain skin elasticity), insulin-like growth factor, platelet-derived factor (eliminate hematopoietic disorders), transforming growth factor, leukocyte elements and anticancer substances. It also contains humoral factors (regulating human...

Claims

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Application Information

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IPC IPC(8): C12P21/02A61K8/98A61K8/64A61K9/70A61Q19/08
Inventor 成勇
Owner YANGZHOU YANGDA LIANHUAN PHARMA GENE ENG
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