Fluidic devices and methods for multiplex chemical and biochemical reactions

Abstract

The present invention describes microfluidic devices that provide novel fluidic structures to facilitate the separation of fluids into isolated, pico-liter sized compartments for performing multiplexing chemical and biological reactions. Applications of the novel devices including biomolecule synthesis, polynucleotide amplification, and binding assays are also disclosed.

Description

technical field [0001] The invention relates to a fluid device capable of performing multiple chemical and biochemical reactions and multiple chemical and biochemical detections. In particular, the invention relates to devices and methods for dispensing and isolating fluids, performing multiple chemical and biochemical reactions, and having the function of detecting multiple chemical and biological products. Background technique [0002] The development of modern medicine, disease diagnosis, pathogen detection, gene discovery and various gene-related technologies and research increasingly rely on the synthesis, screening and detection of a large number of chemical or biochemical products. Traditional methods can only synthesize or detect one substance at a time, which is far from meeting the demand. Therefore, chemical or biochemical reaction systems and devices with high-throughput analysis and synthesis functions are necessary. [0003] At present, the most widely used h...

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Problems solved by technology

However, the fluidic design and principles of operation of the microcard fundamentally limit its miniaturization to the spatial densities already achieved in DNA microarrays (U.S. Patent 6,272,939)

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