Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of breeding pearls by using 6-dimethylin purine to induce triploid jibheader clams

A technology of dimethylaminopurine and spina mussels, which is applied in the field of using 6-dimethylaminopurine to induce triploid spinnae mussels breeding, and achieves the effect of high survival rate

Inactive Publication Date: 2011-05-04
HUNAN UNIV OF ARTS & SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of using 6-DMAP to artificially induce triploid Hypophyllum sinensis and further cultivate pearls has not been reported in the literature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Cultivation of broodlings: Select the original species of Triangle sail mussel with complete shell body, fat shell, smooth shell surface, normal growth, body length 16cm, clam age 5 years old, male to female ratio 1:1, at 18°C-30°C Store for 20 days;

[0028] 2. Sperm and egg collection and fertilization: Select individuals with mature and full gonads, dissect the sperm and eggs, store them in containers filled with filtered water, and filter them through a sieve to remove impurities and tissue fragments; quickly add the eggs to a container filled with 0.005% ammonia water in the container for 5 minutes. Adjust the number of eggs to 10,000 eggs / ml, the water temperature is 18°C-30°C, then add an appropriate amount of sperm and mix well to fertilize;

[0029] 3. Induction treatment: when about 10% of the fertilized eggs have the first polar body, add 6-DMAP inhibitor with a concentration of 50ppm to induce treatment for 60 minutes; then wash the eggs with water sever...

Embodiment 2

[0038] 1. Cultivation of broodlings: Select the original species of Trigonaus mussel with complete body, fat shell, smooth shell surface, normal growth, body length 14cm, age of 4 mussels, male to female ratio 6:1, at 18°C-30°C Store for 30 days;

[0039] 2. Sperm and egg collection and fertilization: Select individuals with mature and full gonads, dissect the sperm and eggs, store them in containers filled with filtered water, and filter them through a sieve to remove impurities and tissue fragments; quickly add the eggs to a container filled with 0.01% ammonia water in the container for 10 minutes. Adjust the number of eggs to 50,000 eggs / ml, the water temperature is 18°C-30°C, then add an appropriate amount of sperm and mix well for fertilization;

[0040] 3. Induction treatment: when about 40% of the fertilized eggs have the first polar body, add 6-DMAP with a concentration of 100ppm for induction treatment for 20 minutes; then wash the eggs with water several times to re...

Embodiment 3

[0049] 1. Cultivation of broodstock: select the original species of Trigonopsis mussel with complete body, fat shell, smooth shell surface, normal growth, body length 18cm, age of 3 mussels, male to female ratio 3:1, at 18°C-30°C Store for 28 days;

[0050] 2. Sperm and egg collection and fertilization: Select individuals with mature and full gonads, dissect the sperm and eggs, store them in containers filled with filtered water, and filter them through a sieve to remove impurities and tissue fragments; quickly add the eggs to a container filled with 0.001% ammonia water in a container for 30 minutes. Adjust the number of eggs to 100,000 eggs / ml, the water temperature is 18°C-30°C, then add an appropriate amount of sperm and mix well for fertilization;

[0051] 3. Induction treatment: when about 90% of the fertilized eggs have the first polar body, add 6-DMAP with a concentration of 200ppm for induction treatment for 40 minutes; then wash the eggs with water several times to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A culture method for inducing triploid Hyriopsis cvmingii Lea by using 6-dimethylamino purine includes such steps as obtaining the ova of triploid Hyriopsis cvmingii Lea and fertilizing with sperm from diploid, using an inhibitor to suppress a first or a second polar body, which is characterized in that the inhibitor is 6-dimethylamino purine for short 6-DMAP. The invention also discloses a methof for culturing pearl by using the triploid Hyriopsis cvmingii Lea. The inhibitor of the invention has characteristics of low cost and toxicity, and adapts to culturing pearl in large water area.

Description

【Technical field】 [0001] The invention relates to a method for artificially cultivating pearls, in particular to a method for using 6-dimethylaminopurine to induce triploid Hypophyllum sinensis to cultivate pearls. 【Background technique】 [0002] With the development of society and the improvement of people's living standards, people pay more and more attention to high-tech agriculture, and the scientificization of breeding industry is becoming more and more important. Especially driven by high profits, the triangular sail clam is the main freshwater pearl-growing species. Inbreeding causes serious germplasm degradation, slowed growth, and severe diseases. It is imperative to renew the mussel species. Triploid mussels have the characteristics of rapid growth, rapid pearl secretion and strong disease resistance. Therefore, it has broad application prospects. The method of using 6-DMAP to artificially induce triploid Hypophyllum sinensis and further cultivate pearls has not ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A61D19/00A61K35/54A61K35/618
CPCY02A40/81
Inventor 杨品红李梦军
Owner HUNAN UNIV OF ARTS & SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com