Nanometre collagen oligopeptide and its producing method
A nano-glue and oligopeptide technology, which is applied in the field of nano-collagen oligopeptides and its preparation, can solve the problems of low skin permeability and no nutritional value of collagen oligopeptides, so as to improve immunity, improve bioavailability, The effect of improving permeability
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Embodiment 1
[0045] Example 1: After the fresh pigskin is depilated, it is scraped, washed, and crushed into small pieces, soaked in 10 times the volume of saturated lime water for 40 days, rinsed with water, washed until nearly neutral, and then washed with 10 times the volume 1M HCl, soaked for 48 hours, washed with water until the pH is equal to about 4, to obtain pretreated pigskin. Take 200g of pretreated pigskin, put it into the reaction bottle of the rotary evaporator, add 1800g of water, add 2g of pepsin and 2g of trypsin respectively, keep a vacuum of 1-10KPa, hydrolyze at a constant temperature of 50°C for 4 hours, and then add 2g of papain , adjusted the pH value to 5 with 0.1M NaOH, and hydrolyzed at 60°C for 3 hours under the same vacuum condition. Then add 2 grams of matrix metalloproteinase, continue to keep constant temperature at 60° C., and hydrolyze under the same vacuum condition for 3 hours. Heat to 100°C for 10 minutes, cool to 60°C, filter with absorbent cotton, and...
Embodiment 2
[0051] Example 2: After the fresh pigskin is depilated, it is scraped, washed, and crushed into small pieces, soaked in 10 times the volume of saturated lime water for 40 days, rinsed with water, washed until nearly neutral, and then washed with 10 times the volume 1M HCl, soaked for 48 hours, washed with water until the pH is equal to about 4, to obtain pretreated pigskin. Take 200g of pretreated pigskin, put it into the reaction bottle of the rotary evaporator, add 1800g of water, add 2g of pepsin and 2g of trypsin respectively, keep a vacuum of 10-30KPa, hydrolyze at a constant temperature of 50°C for 4 hours, and then add 2g of bromelain , adjust the pH value to 5 with 0.1M NaOH, and hydrolyze at 72°C for 3 hours under the same vacuum condition. Then add 2 grams of matrix metalloproteinase, continue to keep constant temperature at 60° C., and hydrolyze under the same vacuum condition for 3 hours. Heat to 100°C for 10 minutes, cool to 60°C, filter with absorbent cotton, an...
Embodiment 3
[0054] Example 3: After the fresh pigskin is depilated, it is scraped off, washed, and crushed into small pieces, soaked in 10 times the volume of saturated lime water for 40 days, rinsed with water, washed until nearly neutral, and then washed with 10 times the volume 1M HCl, soaked for 48 hours, washed with water until the pH is equal to about 4, to obtain pretreated pigskin. Take 200g of pretreated pigskin, put it into the reaction bottle of the rotary evaporator, add 1800g of water, add 2g of pepsin and 2g of trypsin respectively, keep a vacuum of 10-30KPa, hydrolyze at a constant temperature of 40°C for 4 hours, then add 2g of papain , adjusted the pH to 5 with 0.1M NaOH, and hydrolyzed at 70°C for 3 hours under the same vacuum condition. Then add 2 grams of matrix metalloproteinase, continue to keep constant temperature at 60° C., and hydrolyze under the same vacuum condition for 3 hours. Heat to 100°C for 10 minutes, cool to 60°C, filter with absorbent cotton, and then...
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