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Gene delivery system containing relaxin gene and pharmaceutical composition using relaxin

A composition and gene technology, applied in the direction of drug combination, medical preparations containing active ingredients, using vectors to introduce foreign genetic material, etc., can solve the problem of complete eradication of tumors that have not been found

Active Publication Date: 2007-05-23
GENEMEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although administration of recombinant adenovirus induced partial inhibition of tumor growth, complete eradication of the tumor was not found, and regrowth of the tumor occurred rapidly over time

Method used

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  • Gene delivery system containing relaxin gene and pharmaceutical composition using relaxin
  • Gene delivery system containing relaxin gene and pharmaceutical composition using relaxin
  • Gene delivery system containing relaxin gene and pharmaceutical composition using relaxin

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Experimental program
Comparison scheme
Effect test

Embodiment

[0115] Materials and methods

[0116] Cell Lines and Cell Culture

[0117] The cell lines used for the experiments were human brain cancer cell lines (U343, U87MG), cervical cancer cell lines (C33A), liver cancer cell lines (Hep3B), lung cancer cell lines available from the American Type Culture Collection (ATCC) (A549) and the 293 cell line carrying the E1 region of the adenovirus early gene. All cell lines were cultured in Dulbecco's modified Eagle' medium (DMEM; Gibco BRL) supplemented with 10% fetal bovine serum (Gibco BRL), penicillin and streptomycin and maintained at 37 °C for 5 %CO 2 atmosphere.

[0118] Generation and titration of recombinant adenovirus

[0119] To generate a replication-deficient adenovirus expressing relaxin and lac Z as a reporter gene with deletion of the E1 / E3-gene, we first used vmdl324Bst (a gift from Dr. Verca, University of Fribourgh, Switzerland; Heider, H. et al., Biotechniques, 28 (2): 260-265, 268-270 (2000) constructed the pd1-LacZ ...

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Abstract

The present invention relates to a novel gene delivery system and recombinant adenovirus comprising the relaxin-encoding sequence to enhance transduction efficiency of transgenes, a phamaceutical anti-tumor composition comprising the reconvinant adenovirus, a phamaceutical composition having improve tissue penetration potency and a phamaceutical composition for treating a desease or disorder associated with accumulation of excess extarcellular matrix.

Description

technical field [0001] The present invention relates to a novel gene delivery system and a recombinant adenovirus, specifically, the present invention relates to a novel gene delivery system and a recombinant adenovirus comprising a relaxin coding sequence, a recombinant adenovirus comprising the recombinant adenovirus A pharmaceutical antineoplastic composition, a pharmaceutical composition characterized by improved tissue penetration and a pharmaceutical composition useful in the treatment of diseases or conditions associated with the accumulation of excess extracellular matrix. Background technique [0002] Gene therapy aims to treat pathological conditions by introducing foreign genes into cells or tissues. For sickle cell anemia, alpha 1 In the genetic diseases of antitrypsin deficiency, phenylketonuria, hemophilia, and cystic fibrosis, the goal of gene therapy is to replace missing or defective genes in order to make cells or tissues function normally. In addition, g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K38/00A61P35/00
CPCC12N2710/10332C12N15/86A61K48/00A61K38/2221C12N2710/10343A61K48/005A61P1/16A61P11/00A61P13/12A61P17/02A61P35/00A61P43/00A61P9/00A61P9/10H04M1/21C12N2710/10043
Inventor 尹彩钰金周恒
Owner GENEMEDICINE CO LTD
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