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Nano metal chiral identification solid phase, chiral separation chromatograph and its preparation method

A chiral recognition and nano-metal technology, which is applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve problems such as no literature reports, and achieve increased concentration, improved chiral recognition efficiency, and enhanced interaction. Effect

Inactive Publication Date: 2007-05-30
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, there is no literature report on loading chiral recognition agents on nanoparticles as a stationary phase

Method used

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  • Nano metal chiral identification solid phase, chiral separation chromatograph and its preparation method

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Experimental program
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Effect test

Embodiment 1

[0038] The chiral separation chromatographic column using nanoparticles as a stationary phase carrier prepared by the method of the present invention has the following steps:

[0039] 1. Preparation of noble metal (gold) nanoparticles

[0040] In a three-necked flask equipped with a cooling tube, add 100mL of chloroauric acid aqueous solution with a weight percent concentration of 0.01% prepared with pure water, heat to boiling under stirring at 800rm / min, and then add 1% by weight concentration of citric acid three Sodium 3mL, kept boiling for 30 minutes; then naturally cooled to room temperature; filtered through a 0.20μm cellulose acetate microporous membrane to obtain a 10-20nm nano-gold colloidal solution to remove impurities;

[0041] 2. Prepare the conjugate of chiral recognition agent (bovine serum albumin or other proteins) and metal nanoparticles (i.e. nano-metal chiral recognition stationary phase)

[0042] Use 0.1M potassium carbonate to adjust the pH value of the...

Embodiment 2

[0048] 1. Preparation of noble metal (gold) ion nanoparticles:

[0049] Add 100 mL of 0.1% chloroauric acid aqueous solution prepared with pure water into a three-necked flask equipped with a cooling tube, heat and stir at 1400 rm / min until boiling, then add 20 mL of 1% trisodium citrate, and keep boiling for 30 minutes. Then cool to room temperature naturally. Filter the prepared nano-gold colloid solution with a 0.45 μm cellulose acetate microporous membrane to remove impurities. That is, gold nanoparticles with a particle size of less than 50 nm are obtained.

[0050] 2. Preparation of chiral recognition agent-noble metal nanoparticle conjugates:

[0051] The pH value of the nano-gold colloid solution was adjusted to 10 with 0.1 M potassium carbonate. Add the newly prepared 1×10 under stirring at 100rm / min -5 M's neutral digitonin or other saponin aqueous solution (ratio of the amount of nano gold solution and neutral digitonin or other saponin is 120:1). The mixed sol...

Embodiment 3

[0056] 1. Preparation of nano-noble metal (platinum) particles

[0057] Add 100 mL of 0.01% chloroplatinic acid aqueous solution prepared with pure water into a three-necked flask equipped with a condenser, heat to boiling under vigorous stirring, then add 1 mL of 1% trisodium citrate, and keep boiling for 30 minutes. Then cool to room temperature naturally. Filter the prepared nano-platinum colloid solution with a 0.45 μm cellulose acetate microporous membrane to remove impurities. That is, platinum nanoparticles with a particle size of less than 80 nm are obtained.

[0058] 2. Preparation of dextran-gold nanoparticle conjugates (nano metal chiral stationary phase):

[0059] The pH value of the nano-platinum colloid solution was adjusted to 4-5 with 0.1M potassium carbonate. Add the newly prepared 1×10 under stirring at 100rm / min -5 M's dextran (or other saccharide compounds or cyclodextrin compounds) aqueous solution (the ratio of the amount of gold nanometer solution an...

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Abstract

Precious metal ionic nanometer particles and chirality chromatographic fractionation meter starts from making particle solution and metal chirality identification fixed phase fixing it on the capillary tube or micro flow channel through its core plate to get the chirality chromatographic fraction meter. Compared with existing devices, it is simple and feasible in manufacturing, with moderate processing temperature. With highly efficient separation, it shortens the chirality separation period with stability and endurability. It applies to areas that have higher requirements for chirality separation efficiency.

Description

Technical field: [0001] The invention belongs to the field of preparation methods of chiral chromatographic columns, in particular to a nanometer metal chiral recognition stationary phase, chiral separation chromatographic columns and a preparation method. Background technique: [0002] Chiral isomers refer to two chemical compounds whose molecular structures are mirror images of each other but cannot be superimposed. They are called enantiomers. The two chiral molecular formulas are exactly the same, but the spatial orientation of atoms and atomic groups is different. Enantiomers have essentially the same chemical and physical properties in an achiral environment. However, some chiral isomers have huge differences in toxicity, pharmacology and biological metabolism. As far as the field of pharmacy is concerned, as many as 30% to 40% of drugs are chiral. Differences in biological activity or pharmacokinetics of chiral isomers make separation of chiral enantiomers particula...

Claims

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Application Information

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IPC IPC(8): B01J20/29B01J20/30B01D15/38
Inventor 林金明曾湖烈赵利霞王栩
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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