Discrimination of MUC4 mononucleotide polymorphic site with obvious effect on diarrhea resistance of weaned piglet and its application
A single nucleotide polymorphism and weaned piglet diarrhea technology, applied in the fields of application, biochemical equipment and methods, botany equipment and methods, etc., can solve problems such as increased feeding costs, easy to cause drug resistance, drug residues, etc., to achieve The effect of reducing the mortality rate of piglets and improving the ability to resist diarrhea
Inactive Publication Date: 2007-06-06
JIANGXI AGRICULTURAL UNIVERSITY
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Problems solved by technology
Escherichia coli disease seriously affects the growth and development of piglets, leading to increased feeding costs and huge economic losses to the pig industry. Vaccination or antibacterial drugs are used to prevent and treat the disease. On the one hand, long-term use is likely to cause drug resistance. The abuse of animal products will lead to drug residues and become drug food, which will eventually affect human health
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[0029]The pig resource family constructed by itself, with white Duroc and Chinese Erhualian pigs as ancestral parents and used for functional gene mapping, is used as experimental animals. 795 boars of the second generation (F2) of the resource family were raised to 240 days of age and slaughtered. The small intestine and jejunum were taken, and the brush border of small intestinal epithelial cells was extracted. E.coli F4ab, F4ac and F4ad strains were used to adhere to the porcine small intestinal epithelial cell brush. Like edge, the result of bacterial adhesion is detected by phase-contrast microscope. Each sample detects more than 20 brush borders. If more than 10% of the brush borders have at least 2 bacteria attached, the sample is judged to be an adhesive type. If there are at least 2 bacteria attached to the brush borders less than 10 %, but there are more than 10% of the brush-shaped border attached by 1-2 bacteria, the sample is judged to be a weak adhesion type, otherwi...
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The present invention discloses the discrimination and application of MUC4 mononucleotide polymorphic site with obvious effect on diarrhea resistance of weaned piglet and capable of being used in genetic improvement of boar. The present invention includes the first discrimination on mononucleotide polymorphic A243G and the subsequent mononucleotide polymorphic A243G genotype determination in a PCR-Hha I-RFLP process. The present invention detects the polymorphic site by means of modern molecular biological technology, selects favorable allelotype individuals by means of marker aided selection, and can raise the diarrhea resistance of weaned piglet greatly.
Description
Technical field [0001] The present invention relates to a MUC4 single-nucleotide polymorphism site that significantly affects the diarrhea resistance of weaned piglets and can be used for genetic improvement of breeding pigs. Background technique [0002] Livestock and poultry infectious diseases cause huge economic losses to the world's livestock and poultry production every year. The losses caused by this in developed countries account for 17% (1.7 billion pounds) of the annual transaction value, and as high as 35%-50% in developing countries. Piglet diarrhea is the most common and very important disease. The mortality rate of piglets caused by diarrhea is 11.5%-29.5%, among which the morbidity and mortality caused by E. coli account for 56.2% of the morbidity and mortality of pigs. And 24.7%. Colibacillosis seriously affects the growth and development of piglets, leading to higher feeding costs and huge economic losses to the pig industry. Vaccination or antibacterial drugs ar...
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Inventor 黄路生晏学明任军郭源梅麻骏武彭秋玲
Owner JIANGXI AGRICULTURAL UNIVERSITY