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Molecular marker for identifying local pig anti-F4ac piglet diarrhea in China and breeding application

A technology of piglet diarrhea and molecular markers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve problems such as increased feeding costs, decreased sales revenue, and affected growth and development

Active Publication Date: 2011-08-10
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Even if the individual recovers from the disease, it will seriously affect the growth and development, and some will form a dead pig, resulting in an increase in feeding costs and a decrease in sales revenue

Method used

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  • Molecular marker for identifying local pig anti-F4ac piglet diarrhea in China and breeding application
  • Molecular marker for identifying local pig anti-F4ac piglet diarrhea in China and breeding application
  • Molecular marker for identifying local pig anti-F4ac piglet diarrhea in China and breeding application

Examples

Experimental program
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Embodiment

[0052] 148 purebred piglets of 12 local pig breeds representing 6 ecological types in my country (including North China, South China, Central China, Jianghai, Southwest, Plateau, etc.) were used as experimental animals. 3-4 1-2-month-old individuals were selected from each family; 148 purebred piglets were slaughtered, and the jejunum part of the small intestine was taken to extract the brush border of small intestinal epithelial cells, and the E.coli F4ac strain was used to adhere to the brush border of pig small intestinal epithelial cells. Bacterial adhesion results were detected by phase-contrast microscopy. More than 20 brush borders were detected for each sample. If at least 2 bacteria adhered to more than 10% of the brush borders, the sample was judged as adherent type. If at least 2 bacteria adhered to the brush borders, less than 10 %, but there are more than 10% of brush borders with 1-2 bacterial adhesion, the sample is judged as weak adhesion type, otherwise it is ju...

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Abstract

The invention discloses a molecular marker for identifying F4ac diarrhea affectability / resistance of newborn and weaned piglets of local pig breeds in China and application thereof in boar genetic improvement of the local pig breeds in China, wherein by cloning, a DNA segment as shown in a sequence table is obtained, a single nucleotide polymorphism (SNP) A183G is identified at a 183bp part of the sequence, and an SNaPshot method is adopted to perform genotyping on the SNP A183G. The invention also discloses a preparation method for obtaining the molecular marker and related primers and a method for performing diarrhea trait association analysis of local pigs in China by using the cloned molecular marker. The molecular marker and the methods can remarkably improve the ability of resistingdiarrhea diseases of the weaned piglets of local pig breed population in China and greatly reduce the piglet mortality by using the modern molecular biology technique to detect polymorphic sites and using marker-assisted selection (MAS) to select advantageous genotype individuals having seed stocks.

Description

technical field [0001] The invention relates to the field of animal molecular biology technology and breeding, in particular to a molecular marker for identifying susceptibility / resistance to F4ac diarrhea of ​​newborn and weaned piglets of Chinese local pig breeds and its application in genetic improvement and breeding of Chinese local pig breeds. Background technique [0002] Piglet diarrhea is a common infectious disease in pig production, which has caused huge economic losses to the world's pig industry. A large-scale field survey of the Danish pig industry showed that 6%-7% of newborn piglets suffered from diarrheal disease, causing the mortality rate of piglets before weaning to reach 2.7%, accounting for 11.9% of the entire mortality rate during this period. Even the recovered individual will seriously affect the growth and development, and some will form dead pigs, resulting in increased feeding costs and decreased sales revenue. [0003] Enterotoxigenic Escherichia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 黄路生任军晏学明艾华水
Owner JIANGXI AGRICULTURAL UNIVERSITY