Tissue paraffin section de-waxing method

A paraffin section and tissue technology, applied in the preparation of test samples, etc., can solve problems such as adverse effects on the human body and the environment, xylene pollution of the environment, and human harm, and achieve simple and easy operation, clear dewaxing effect, and low cost. cheap effect

Inactive Publication Date: 2007-06-06
NANKAI UNIV
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this dewaxing method, not only the operation is cumbersome, but multiple steps are required, and xylene pollutes the environment and is harmful to the human body.
However, in order to improve this situa

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0015] Pour the dewaxing solution prepared by the above method into two glass staining jars, and put them in a water bath for preheating. Tissue paraffin sections were soaked in one of the jars, and soaked at a constant temperature of 60°C for 10 minutes. After the tissue slices were directly taken out, they were placed in another jar and soaked at a constant temperature of 60°C for 10 minutes. The tissue sections were then removed from the solution and rinsed with tap water. The steps of hematoxylin-eosin staining, conventional dehydration, and neutral gum mounting can be performed according to conventional methods.

[0016] Make the dewaxing liquid into the dewaxing liquid for antigen retrieval, and repair it with the antigen thermal repair box, the method is the same as the antigen thermal repair after dewaxing the paraffin section of the general tissue. After the tissue section is taken out of the solution, it can be washed with tap water. Immunohistochemical staining, r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention disclosed a de-waxing method used for paraffin-cut section. De-waxing solution was prepared by mixing distilled water and Triton X-100 by volume ratio of 99:1. It can be used by the following procedure: place the slice into the first de-wax solution and dip for 10-2 minutes at 60-70DEG C; take it out and place it to the second de-wax solution and repeat the above operation; wash it by water. The solution Triton X-100 used in this invention was safe, cost save, environmental friendly and it's easy to handle.

Description

【Technical field】 [0001] The invention relates to the technical field of pathological tissue sections, in particular to a method for dewaxing tissue paraffin sections with the characteristics of simplicity, environmental protection and low cost. 【Background technique】 [0002] Tissue paraffin section is the most commonly used sectioning technique in qualitative analysis such as observation of tissue morphology and immunohistochemistry, and is widely used in clinical pathological diagnosis and experimental research. In order to observe the tissue, the paraffin of the paraffin section must first be removed, that is, deparaffinization. The conventional dewaxing method for tissue paraffin sections is to use organic solvents, for example, xylene is a common dewaxing agent. In this dewaxing method, not only the operation is cumbersome and multiple steps are required, but also the xylene pollutes the environment and is harmful to the human body. However, in order to improve this ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/28
Inventor 王腾飞车永哲
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap