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Tissue paraffin section de-waxing method

A paraffin section and tissue technology, applied in the preparation of test samples, etc., can solve problems such as adverse effects on the human body and the environment, xylene pollution of the environment, and human harm, and achieve simple and easy operation, clear dewaxing effect, and low cost. cheap effect

Inactive Publication Date: 2007-06-06
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this dewaxing method, not only the operation is cumbersome, but multiple steps are required, and xylene pollutes the environment and is harmful to the human body.
However, in order to improve this situation, so far, the improved dewaxing methods still mostly use organic solvents as dewaxing agents, or use detergent solution for dewaxing, but there are still adverse effects on human body and environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0015] Pour the dewaxing solution prepared by the above method into two glass staining jars, and put them in a water bath for preheating. Tissue paraffin sections were soaked in one of the jars, and soaked at a constant temperature of 60°C for 10 minutes. After the tissue slices were directly taken out, they were placed in another jar and soaked at a constant temperature of 60°C for 10 minutes. The tissue sections were then removed from the solution and rinsed with tap water. The steps of hematoxylin-eosin staining, conventional dehydration, and neutral gum mounting can be performed according to conventional methods.

[0016] Make the dewaxing liquid into the dewaxing liquid for antigen retrieval, and repair it with the antigen thermal repair box, the method is the same as the antigen thermal repair after dewaxing the paraffin section of the general tissue. After the tissue section is taken out of the solution, it can be washed with tap water. Immunohistochemical staining, r...

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PUM

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Abstract

This invention disclosed a de-waxing method used for paraffin-cut section. De-waxing solution was prepared by mixing distilled water and Triton X-100 by volume ratio of 99:1. It can be used by the following procedure: place the slice into the first de-wax solution and dip for 10-2 minutes at 60-70DEG C; take it out and place it to the second de-wax solution and repeat the above operation; wash it by water. The solution Triton X-100 used in this invention was safe, cost save, environmental friendly and it's easy to handle.

Description

【Technical field】 [0001] The invention relates to the technical field of pathological tissue sections, in particular to a method for dewaxing tissue paraffin sections with the characteristics of simplicity, environmental protection and low cost. 【Background technique】 [0002] Tissue paraffin section is the most commonly used sectioning technique in qualitative analysis such as observation of tissue morphology and immunohistochemistry, and is widely used in clinical pathological diagnosis and experimental research. In order to observe the tissue, the paraffin of the paraffin section must first be removed, that is, deparaffinization. The conventional dewaxing method for tissue paraffin sections is to use organic solvents, for example, xylene is a common dewaxing agent. In this dewaxing method, not only the operation is cumbersome and multiple steps are required, but also the xylene pollutes the environment and is harmful to the human body. However, in order to improve this ...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 王腾飞车永哲
Owner NANKAI UNIV
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