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Sex identification method for silkworm larva

A silkworm and gender technology, applied in the field of sex identification of silkworm larvae, can solve the problem that silkworm larvae cannot be identified well

Inactive Publication Date: 2007-07-04
上海市向明初级中学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method for identifying the sex of silkworm larvae using DNA gene method, so as to solve the problem that existing silkworm larvae cannot be well identified

Method used

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  • Sex identification method for silkworm larva

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Experimental program
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Embodiment Construction

[0020] Extraction and DNA Determination of Bombyx mori

[0021] First, take a small amount of tissue from the silkworm for DNA extraction. After diluting the extract in a certain ratio, measure the OD of the extract with a UV spectrophotometer at a wavelength of 260nm to estimate the approximate concentration. Then take 1-3 μl of the extract for agarose gel electrophoresis Figure 1, band 100 is Marker, band 101-117 is the total DNA of silkworm extracted, and 101-112 is the total DNA of silkworm that has not been purified or enzymatically digested. DNA, 113-117 is the total DNA band of silkworm after purification and digestion of RNA. The band 120 located near the sample wells 101-117 is the total DNA of the silkworm. The diffuse band 121 located at the far end of the sample wells 101-112 is RNA.

[0022] PCR amplification and detection of specific genes

[0023] Utilize the genes that control silkworm sex, such as the white egg gene sequence that is known to obtain sex-limi...

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Abstract

The invention provides a method for identifying sex for silkworm larvae. It comprises: extracting DNA from silkworm larvae, testing nucleic acid by using spectrophotometer and agarose gel electrophoresis; designing inducer by using gentic sequence relative to factor deciding the silkworm larvae sex, carrying out PCR amplification; agarose gel electrophoresis for PCR amplification; identifying the sex according to result got from electrophoresis. The method is easy to operate and can increase the producing benefit for owners.

Description

technical field [0001] The invention relates to a method for identifying the sex of organisms, in particular to a method for identifying the sex of silkworm larvae. Background technique [0002] Planting mulberry and raising silkworms originated in my country and has a history of more than 5,000 years. The cocoon silk industry has always been a dominant traditional industry in my country. The annual output and export volume of cocoon raw silk account for more than 70% and 80% of the world's total respectively, and it is the center of the world's cocoon silk industry. [0003] However, in the 100 years of the 20th century, the sericulture technology system adopted by our country was generally based on the establishment of foreign countries in the 1930s and 1940s, and there was no obvious breakthrough in sericulture technology for a long period of time. [0004] Based on modern science and technology, the silkworm breeding technology system carried out by using biological gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 汤霈霈
Owner 上海市向明初级中学
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