Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner

Inactive Publication Date: 2007-02-15
DE CRECY EUDES FRANCOIS MARIE
View PDF8 Cites 29 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0016] Continuous culture is achieved inside a flexible sterile tube filled with growth medium. The medium and the chamber surface are static with respect to each other, and both are regularly and simultaneously replaced by peristaltic movement of the tubing through “gates”, or points at which the tube is sterilely subdivided by clamps that prevent the cultured cells from moving between regions of the tube. UV gates can also (optionally) be added upstream and downstream of the culture vessel for additional security.
[0017] The present method and device are also an improvement over prior art insofar as they continually, rather than periodically, select against adherence of dilution-resistant variants to the chemostat surfaces, as replacement

Problems solved by technology

This process is usually done manually, with considerable labor investment, and is subject to contamination through exposure to the outside environment.
Serial culture is also inefficient, as described in the following paragraph.
Traditional chemostats are unable to sustain long periods of selection for increased reproduction rate, due to the unintended selection of dilution-resistant (static) variants.
This method and device achieves the goal, but requires independent comple

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner
  • Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner
  • Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0048] The basic operation of the device is depicted in FIGS. 3 through 9.

[0049] One potential configuration for the present device is shown in FIG. 1, as it appears after having been loaded with a fresh tube of sterile medium (shown divided into regions A-H by said gates (3), (4) and (5)).

[0050] Inoculation of the device with the chosen cell could be achieved by introduction of the cell into the growth chamber (FIG. 3), through injection (FIG. 4, region B). The culture would then be allowed to grow to the desired density and continuous culture would begin (FIG. 5).

[0051] Continuous culture would proceed by repetitive movements of the gated regions of tubing. This involves simultaneous movements of the gates, the tubing, the medium, and any culture within the tubing. The tubing will always move in the same direction; unused tubing containing fresh medium (and hereafter said to be ‘upstream’ of the growth chamber (7)) will move into the growth chamber and mix with the culture rema...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method and device for growing plant, animal or stem cells in a continuous manner.

Description

FIELD OF THE INVENTION [0001] The described invention provides a method and a device that allow selection of living cells, with increased rates of reproduction and specific metabolic properties, in a liquid or semi-solid medium. For the process of selection (adaptive evolution), genetically variant organisms (mutants) arise in a population and compete with other variants of the same origin. Those with the fastest rate of reproduction increase in relative proportion over time, leading to a population (and individual organisms) with increased reproductive rate. This process can improve the performance of organisms used in industrial processes or academic purpose. The present invention utilizes a continuous culture apparatus to achieve the viable production of living cells, for example, yeast, plant cells, animal cells or stem cells. The present invention may be used to produce an active ingredient or biologic that is produced by the living cells. The active ingredient or biologic may ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M1/00
CPCC12M23/06C12N5/0606C12M23/34C12M41/26C12M41/36C12M41/40C12N1/16C12N5/0673C12N5/0647C12N5/0622C12N7/00C12N5/0605C12N5/04C12N1/20C12M23/26C12N5/0669C12N5/0602C12M1/007C12M1/02C12M1/24C12M1/3453C12M3/00C12M3/02C12M3/06C12M23/22C12M23/24
Inventor DE CRECY, EUDES FRANCOIS MARIE
Owner DE CRECY EUDES FRANCOIS MARIE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap