Tumor markers in ovarian cancer

a tumor marker and ovarian cancer technology, applied in the field of identification of ovarian tumor markers, can solve the problems of low survival rate of ovarian cancer patients, difficult detection of ovarian cancer, and early stages

Inactive Publication Date: 2008-10-16
MORIN PATRICE J +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ovarian cancer is difficult to detect in its early stages, and remains the leading cause of death among women with cancer of the female reproductive tract.
The low survival rate of ovarian cancer patients is in part due to the lack of good diagnostic markers for the detection of early stage neoplasms, and in part due to a deficit in the general understanding of ovarian cancer biology, which would facilitate the development of effective anti-tumor therapies.

Method used

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  • Tumor markers in ovarian cancer

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example i

Identification of Ovarian Tumor Marker Genes Using Sage

[0096]Serial Analysis of Gene Expression is a method that enables the global analysis of gene expression from a tissue of interest (Velculescu et al., Science 270:484-487, 1995; Zhang et al., Science 276:1268-72, 1997). The advantages of SAGE over cDNA arrays, another method for the global analysis of gene expression, include: 1) the possibility of identifying novel genes, 2) determination of absolute levels of gene expression, which is difficult in hybridization-based techniques, and, 3) examination of gene expression as a whole instead of as a subset of genes.

[0097]Construction and Screening of SAGE Libraries

[0098]The SAGE technique has been described in detail (Velculescu et al., Science 270:484-487, 1995). The SAGE libraries disclosed herein were made as described by Velculescu, supra. First, total RNA was purified from the cells. Poly A+ RNA was then isolated and reverse transcription was performed using a biotinylated poly...

example ii

Identification of Additional Ovarian Tumor Marker Genes Using Sage

[0107]Serial Analysis of Gene Expression (SAGE) was used to generate global gene expression profiles from various ovarian cell lines and tissues, including primary cancers, ovarian surface epithelial (OSE) cells and cystadenoma cells. The profiles were used to compare overall patterns of gene expression and identify differentially expressed genes. We have sequenced a total of 385,000 tags, yielding over 56,000 genes expressed in ten different libraries derived from ovarian tissues.

[0108]In general, ovarian cancer cell lines showed relatively high levels of similarity to libraries from other cancer cell lines, regardless of the tissue of origin (ovarian or colon), indicating that these lines had lost many of their tissue specific expression patterns. In contrast, immortalized OSE (IOSE) and ovarian cystadenoma cells showed much higher similarity to primary ovarian carcinomas as compared to primary colon carcinomas. Pri...

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Abstract

The present invention features methods of diagnosing and prognosticating ovarian tumors by detecting increased expression of an ovarian tumor marker gene in a subject or in a sample from a subject. Also featured are kits for the aforementioned diagnostic and prognostic methods. In addition, the invention features methods of treating and preventing ovarian tumors, and methods of inhibiting the growth or metastasis of ovarian tumors, by modulating the production or activity of an ovarian tumor marker polypeptide. Further featured are methods of inhibiting the growth or metastasis of an ovarian tumor by contacting an ovarian tumor cell with an antibody that specifically binds an ovarian tumor marker polypeptide.

Description

[0001]This application is a continuation of U.S. application Ser. No. 10 / 257,021 which is the National Stage of International Application No. PCT / US01 / 10947, filed on Apr. 3, 2001, which claims the benefit of U.S. Provisional Application 60 / 194,336, filed on Apr. 3, 2000, which are hereby incorporated by reference in their entirety.[0002]This invention was made with intramural support from the National Institutes of Health. The government has certain rights in the invention.FIELD OF THE INVENTION[0003]This invention relates generally to the identification of ovarian tumor markers and diagnostic, prognostic, and therapeutic methods for their use, as well as kits for use in the aforementioned methods.BACKGROUND OF THE INVENTION[0004]Ovarian cancer is one of the most common forms of neoplasia in women. Early diagnosis and treatment of any cancer ordinarily improves the likelihood of survival. However, ovarian cancer is difficult to detect in its early stages, and remains the leading ca...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K51/10A61K39/395A61P35/04C12Q1/68C12Q1/6809C12Q1/6886
CPCC12Q1/6809C12Q1/6886C12Q2600/158A61P35/00A61P35/04
Inventor MORIN, PATRICE J.SHERMAN-BAUST, CHERYL A.PIZER, ELLEN S.HOUGH, COLLEEN D.
Owner MORIN PATRICE J
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