Universal sample preparation system and use in an integrated analysis system

a sample preparation and integrated analysis technology, applied in electrostatic separators, diaphragms, electrolysis, etc., can solve the problem of ubiquitous sample preparation, and achieve the effect of easy mixing and manipulation and adaptability to microscal

Inactive Publication Date: 2009-10-08
INTEGENX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sample preparation is a ubiquitous problem in biological analytical systems.

Method used

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  • Universal sample preparation system and use in an integrated analysis system
  • Universal sample preparation system and use in an integrated analysis system
  • Universal sample preparation system and use in an integrated analysis system

Examples

Experimental program
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example 1

Operation of a Cartridge for Nucleic Acid Purification

[0229]This example refers to the use of a device comprising a cartridge mated to a microchip. The numbers refer to the cartridge of FIG. 3 and FIG. 4 mated to a microchip with the circuit architecture of FIG. 5. This sub-assembly also can be fluidically connected other sub-assemblies in the instrument of FIG. 6. For reference, a cartridge mated with a microchip also is shown in FIG. 40 and FIG. 59.

[0230]Nucleic acids can be purified from a wide variety of matrices for many purposes including, but not limited to, genotyping, identification, forensics, gene expression, gene modification, microRNA analysis, ribotyping, diagnostics, or therapeutics. The input sample can be a solid, swab, liquid, slurry, aerosol or a gas.

[0231]For molecular diagnostics and forensics, swabs are commonly used. A buccal swab can be taken using a swab with an ejectable tip and the swab ejected into a syringe attached to connection 7 of FIG. 4. Connection ...

example 2

Universal Sample Preparation

[0280]The previous example illustrated one embodiment in which the disclosed apparatus can be used to prepare samples for analysis and showed one example of STR amplification. Another embodiment involves the use of a Universal Sample Preparation Module (USPM). The USPM device can consist of a sample processing cartridge (1), accompanying apparatus to operate the cartridge, a microprocessor, and software that can readily be interfaced to downstream analytical devices. In one embodiment the USPM can be tightly integrated with analytical devices to form a modular sample-to-answer system. The cartridge can be configured as a disposable single-use device that can process swabs or liquids (including aerosol samples) for field monitoring processes, or as a reusable, flow-through format for remote operations with rare positives. Target specificity of the USPM is imparted through the use of specific antibodies (that bind selected targets) attached to paramagnetic ...

example 3

Sample Preparation for Library Construction

[0298]The cartridge (1) technology and the sample preparation device can be further used to perform a series of complex molecular biology reactions in small volumes with bead-based manipulation of DNA samples. The DNA can be processed to prepare genomic libraries for next generation sequencing systems (i.e., Roche 454 system, or the Applied Biosystems SOLiD), Real-time PCR, or other DNA assay systems.

[0299]By incorporating a reverse transcriptase step, RNA libraries can also be converted into DNA libraries by essentially the same method. The advantage of building an RNA library is that the representation in the final amplified library will directly mirror the original starting material since the amplification will be based upon single molecule amplification.

[0300]The design concept for the Library Construction Module (LCM) is an instrument that holds bulk reagents and provides fluidic control to the small disposable Library Construction Mod...

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Abstract

The invention provides for devices and methods for interfacing microchips to cartridges and pneumatic manifolds. The cartridges, microchips, and pneumatic manifolds can be integrated with downstream preparation devices, such as thermal regulating devices and separation and analysis devices.

Description

CROSS-REFERENCE[0001]This application claims the benefit of U.S. Provisional Application No. 61 / 022,722, filed Jan. 22, 2008 and U.S. Provisional Application No. 61 / 140,602, filed Dec. 23, 2008, which are hereby incorporated by reference in their entirety.STATEMENT AS TO FEDERALLY SPONSORED RESEARCH[0002]Aspects of this invention were made with government support under one or more of Project No. W911SR-04-P-0047 awarded by the Department of Defense and Grant No. 5R01HG003583 awarded by the NIH. The government may have certain rights in this invention.BACKGROUND OF THE INVENTION[0003]Sample preparation is a ubiquitous problem in biological analytical systems. The issue of providing sufficiently purified targets from diverse raw sample types to reliably perform downstream analytical assays is pervasive and covers cell biology, genomics, proteomics, metabolomics, food biology, molecular diagnostics, and many other biological and medical assays. While many advances in sample preparation...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12P19/34B01D17/06B01D57/02B01L3/00G01N33/00C12M1/00G01N1/18
CPCB01L2300/087Y10T436/143333B01L2300/1827B01L2400/043G01N1/40G01N27/44791G01N35/1095G01N2035/00158B01D17/06B01L3/50273B01L7/52B01L2200/027B01L2200/04B01L2200/0647B01L2200/10B01L2300/0819G01N1/34Y10T436/25375B01L2300/1822B01L3/502738C12Q1/6837G01N1/18
Inventor VANGBO, MATTIASNIELSEN, WILLIAM D.BLAGA, LULLU I.STERN, SETHHORN, JOANNENGUYEN, MICHAEL VANJOVANOVICH, STEVAN B.
Owner INTEGENX
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