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Substrate for Biochip, Biochip, Method for Manufacturing Substrate for Biochip and Method for Manufacturing Biochip

a biochip and substrate technology, applied in the field of biochip detection techniques, can solve the problems of reducing the detection accuracy of the dna chip, difficult to fix the probe dna only in the specific location, and becoming background nois

Inactive Publication Date: 2010-09-16
YAMATAKE HONEYWELL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a substrate for a biochip that includes a base plate, a metallic membrane, and a crosslinkable polymer membrane. The substrate can also have a plurality of probe biomolecules bonded to it. The invention also provides methods for manufacturing the substrate and a biochip that includes the substrate. The technical effects of the invention include improved accuracy and efficiency in the manufacturing process for biochip production, as well as improved performance and reliability of the resulting biochip.

Problems solved by technology

However, it is difficult to fix the probe DNA only in the specific location.
Therefore, the target DNA bonded to the probe DNA fixed in location other than the specific location becomes background noise in a detection process.
It becomes a factor to decrease a detection accuracy of the DNA chip.

Method used

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  • Substrate for Biochip, Biochip, Method for Manufacturing Substrate for Biochip and Method for Manufacturing Biochip

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Experimental program
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Effect test

first embodiment

[0071]With reference to FIG. 1 and FIG. 2 that is a sectional view taken on line II-II, a substrate for a biochip according to a first embodiment of the present invention includes a base plate 15 having a surface on which a plurality of hydroxyl groups (—OH) can be introduced, and a metallic membrane 13 disposed on the base plate 15, wherein a plurality of wells 41a, 41b, 41c, 41d, 41e, 41f, 41g, 41h, 41i, 42a, 42b, 42c, 42d, 42e, 42f, 42g, 42h, 42i, 43a, 43b, 43c, 43d, 43e, 43f, 43g, 43h, 43i, 44a, 44b, 44c, 44d, 44e, 44f, 44g, 44h, 44i, 45a, 45b, 45c, 45d, 45e, 45f, 45g, 45h, 45i, 46a, 46b, 46c, 46d, 46e, 46f, 46g, 46h, 46i, 47a, 47b, 47c, 47d, 47e, 47f, 47g, 47h, 47i, 48a, 48b, 48c, 48d, 48e, 48f, 48g, 48h, 48i, 49a, 49b, 49c, 49d, 49e, 49f, 49g, 49h, 49i that reach the base plate 15 are delineated in the metallic membrane 13. The film thickness of the base plate 15 is 550 micrometers, for example. Synthetic quarts (SiO2) and a silicon substrate having a surface on which a natura...

second embodiment

[0079]With reference to FIG. 7 and FIG. 8 that is a sectional view taken on line VIII-VIII, the biochip according to a second embodiment of the present invention includes the base plate 15, and the metallic membrane 13 disposed on the base plate 15, wherein the plurality of wells 41a, 41b, 41c, 41d, 41e, 41f, 41g, 41h, 41i, 42a, 42b, 42c, 42d, 42e, 42f, 42g, 42h, 42i, 43a, 43b, 43c, 43d, 43e, 43f, 43g, 43h, 43i, 44a, 44b, 44c, 44d, 44e, 44f, 44g, 44h, 44i, 45a, 45b, 45c, 45d, 45e, 45f, 45g, 45h, 45i, 46a, 46b, 46c, 46d, 46e, 46f, 46g, 46h, 46i, 47a, 47b, 47c, 47d, 47e, 47f, 47g, 47h, 47i, 48a, 48b, 48c, 48d, 48e, 48f, 48g, 48h, 48i, 49a, 49b, 49c, 49d, 49e, 49f, 49g, 49h, 49i that reach the base plate 15 are delineated in the metallic membrane 13. Each film thickness and each material of the base plate 15 and the metallic membrane 13 are similar to the substrate for the biochip shown in FIG. 1. So, an explanation is omitted.

[0080]With reference to FIG. 8, the biochip according to th...

third embodiment

[0105]With reference to FIG. 30 and FIG. 31 that is a sectional view taken on line XXXI-XXXI, a substrate for a biochip according to a third embodiment includes the base plate 15 having the surface on which the plurality of hydroxyl groups can be introduced, and a cover member 211 disposed on the base plate 15 when the probe biomolecules are bonded to the plurality of hydroxyl groups, and having a plurality of through holes 141a, 141b, 141c, 141d, 141e, 141f, 141g, 141h, 141i, 142a, 142b, 142c, 142d, 142e, 142f, 142g, 142h, 142i, 143a, 143b, 143c, 143d, 143e, 143f, 143g, 143h, 143i, 144a, 144b, 144c, 144d, 144e, 144f, 144g, 144h, 144i, 145a, 145b, 145c, 145d, 145e, 145f, 145g, 145h, 145i, 146a, 146b, 146c, 146d, 146e, 146f, 146g, 146h, 146i, 147a, 147b, 147c, 147d, 147e, 147f, 147g, 147h, 147i, 148a, 148b, 148c, 148d, 148e, 148f, 148g, 148h, 148i, 149a, 149b, 149c, 149d, 149e, 149f, 149g, 149h, 14′9i defining biding areas of the probe biomolecules on the surface of base plate 15.

[01...

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Abstract

A base plate having a surface on which a plurality of hydroxyl groups can be introduced, a metallic membrane disposed on the base plate and having a plurality of wells reaching the base plate, and a crosslinkable polymer membrane disposed on the metallic membrane are included.

Description

TECHNICAL FIELD[0001]This invention relates to a technique for detecting a biomolecule, especially to a substrate for a biochip, the biochip, a method for manufacturing the substrate for the biochip, and a method for manufacturing the biochip.BACKGROUND ART[0002]“Biochip” is the generic term for devices in which probe biomolecules that react with to-be-detected target biomolecules in a specific manner are fixed at predetermined positions on a chip surface. A deoxyribonucleic acid (DNA) chip that is a typical example of the biochip is used to detect the types and amounts of target DNA included in blood or cell extract. The DNA chip has, for example, a structure in which thousands to tens of thousands of types of probe DNA, each being single-chain DNA having a known sequence, are arranged in an array on a substrate such as a glass slide.[0003]When a to-be-examined liquid containing fluorescence-marked target DNA is supplied to the DNA chip, only the target DNA which has sequences comp...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): B32B3/10B32B27/38B32B17/10B44C1/22
CPCB01L3/5025B01L2300/0636Y10T428/24331G01N33/54386G01N33/54393G01N33/54353Y10T428/31529Y10T428/31525B01J19/0046B01J2219/00675B01J2219/00729B01J2219/00626
Inventor GOSHOO, YASUHIROKUROIWA, TAKAAKIISHIKAWA, NAOHIROOBARA, DAISUKEYAMASAKI, SHINSUKESASAKI, KAZUKOHORIGUCHI, YASUKO
Owner YAMATAKE HONEYWELL CO LTD