System For Multi Color Real Time PCR

a multi-color, real-time pcr technology, applied in the field of real-time pcr, can solve the problems of no respective successful application protocol published, no system disclosed for performing multi-pex experiments with a higher degree of complexity, and the design of appropriate fret hybridization probe sequences may sometimes be limited
US20110151550A1Inactive Publication Date: 2011-06-23ROCHE MOLECULAR SYST INC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
ROCHE MOLECULAR SYST INC
Publication Date
2011-06-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention is directed to a system for performing multi-color real time PCR, comprising a flexible real time PCR instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
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Description

FIELD OF INVENTION

[0001] The present invention relates to the field of Real time PCR. In particular, the present invention is directed to a system for performing multiplex real time PCR.PRIOR ART BACKGROUND

[0002] Amplification of DNA by polymerase chain reaction (PCR) is a technique fundamental to molecular biology. Nucleic acid analysis by PCR requires sample preparation, amplification, and product analysis. Although these steps are usually performed sequentially, amplification and analysis can occur simultaneously. DNA dyes or fluorescent probes can be added to the PCR mixture before amplification and used to analyze PCR products during amplification. Sample analysis occurs concurrently with amplification in the same tube within the same instrument. This combined approach decreases sample handling, saves time, and greatly reduces the risk of product contamination for subsequent reactions, as there is no need to remove the samples from their closed containers for further analysis. Th...

Claims

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