Composition for diagnosing parkinson's disease containing adipose tissue-derived mesenchymal stromal cell

a technology of mesenchymal stromal cells and parkinson's disease, which is applied in the field of composition for diagnosing parkinson's disease containing mesenchymal stromal cells, can solve the problems of inaccuracy of analysis, risk to patients from directly taking brain tissue samples, and unwanted pain, so as to achieve accurate diagnosis of parkinson

Inactive Publication Date: 2011-12-22
SEOUL NAT UNIV R&DB FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0013]To solve the problems in the art, the present inventors found that Parkinson's disease could be diagnosed by using the mesenchymal stromal cell derived from adipose tissue, developed a technolo...

Problems solved by technology

However, such methods still lead to inaccurate analysis of results, and unw...

Method used

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  • Composition for diagnosing parkinson's disease containing adipose tissue-derived mesenchymal stromal cell
  • Composition for diagnosing parkinson's disease containing adipose tissue-derived mesenchymal stromal cell
  • Composition for diagnosing parkinson's disease containing adipose tissue-derived mesenchymal stromal cell

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example 1

Preparation of the Mesenchymal Stromal Cell Derived from the Adipose Tissue

[0072]1.1. Separation and Culture of the Mesenchymal Stromal Cells Derived from the Adipose Tissue

[0073]The adipose tissue-derived mesenchymal stromal cells (hAD-MSC) were separated from Idiopathic Parkinson's disease (Idiopathic PD) patient, Parkin-deficient Parkinson's disease (Parkin-deficient PD) patient, and a pituitary adenoma patient who did not suffer from Parkinson's disease, and then subsequently were cultured. Hereinafter, otherwise particularly defined, hAD-MSC obtained from Idiopathic PD patient is referred to as “PD,” hAD-MSC obtained from Parkin-deficient Parkinson's disease patient to as “Parkin”, and hAD-MSC obtained from Parkinson's disease to as “non-PD” or “PA.”

[0074]These tests were performed under the permission of Institutional Review Board of Seoul National University Hospital (IRB No. 0707-024-212) and the written consent of the patients. During the performance of Deep Brain Stimulati...

example 2

Gene Profiling of Adipose Tissue-Derived Mesenchymal Stromal Cell

[0093]2.1. cDNA Microarray Analysis

[0094]The gene expression analysis was performed with Affymetrix GeneChip® Human Gene 1.0 ST oligonucleotide array (DNA LINK, INC (Seoul, Korea)).

[0095]According to Affymetrix manufactor's protocol (http: / / www.affymetrix.com), 300 ng of RNA was added to each sample. Namely, 300 ng of all RNA per a sample was changed into double stranded cDNA. The double stranded cDNA was obtained by using SuperScrpit II Reverse Transcriptase, DNA polymerase I and random hexamer inserted by T7 promoter (Affymetrix GeneChip® WT cDNA Synthesis and Amplipicaition Kit, Cat No. 900672). An amplified RNA (cDNA) was produced by in vitro transcription (IVT) with IVT Enzyme Mix (Affymetrix GeneChip® WT cDNA Synthesis and Amplipicaition Kit, Cat No. 900672), and separated with Affymetrix sample cleanup module. The amplified cRNA was mixed with IVT cRNA binding buffer and 100% EtOH, and bonded in cRNA cleanup spi...

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Abstract

The present invention relates to a composition for diagnosing a Parkinson's disease comprising mesenchymal stromal cells derived from adipose tissue, a method of providing information for diagnosing Parkinson's disease and/or the extent of the disease progression, a biomarker for diagnosing a Parkinson's disease, and a method of screening a drug candidate treating Parkinson's disease where the drug candidate is a target of the biomarker.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application claims priority to and the benefit of Korean Patent Application No. 10-2010-0058273 filed in the Korean Intellectual Property Office on Jun. 18, 2010, the entire contents of which are incorporated herein by reference.BACKGROUND OF THE INVENTION[0002](a) Field of the Invention[0003]The present invention relates to a composition for diagnosing a Parkinson's disease comprising mesenchymal stromal cells derived from adipose tissue, a method of providing information for diagnosing Parkinson's disease and / or extent of disease progression using mesenchymal stromal cells derived from adipose tissue, a biomarker for diagnosing a Parkinson's disease, and a method of screening an agent treating Parkinson's disease where the agent targets the biomarker.[0004](b) Description of the Related Art[0005]Parkinson's disease (PD) is a secondarily common neurodegenerative disease which about one percent of old people aged over 60 suffers from,...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N5/077
CPCC12Q1/6883C12Q2600/158C12Q2600/136G01N2800/2835G01N33/5023C12N5/0667C40B40/08G01N33/15C12N5/0653C12N2510/04
Inventor PAEK, SUN HAMOON, HYO EUNPARK, HYUNG WOOSHIN, HYE YOUNG
Owner SEOUL NAT UNIV R&DB FOUND
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