Immunogenic composition and uses thereof

Inactive Publication Date: 2012-03-15
UNIVERSITY OF MELBOURNE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0011]In a third aspect the present invention provides a method of raising an immune response in a subject, the method

Problems solved by technology

In the case of viral infections and in many cases of cancer, antibody is of limited benefit in recovery and th

Method used

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  • Immunogenic composition and uses thereof
  • Immunogenic composition and uses thereof
  • Immunogenic composition and uses thereof

Examples

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Example

Example 1

Introduction

[0050]Protein antigens, particularly recombinant proteins, are often not immunogenic and formulation with adjuvant is necessary to enhance their immunogenicity although concerns about adjuvant toxicity and their mechanism of action need to be resolved before they can be licensed for use in humans. Therefore the development of novel systems that can facilitate the delivery of protein antigens by directly targeting and concurrently activating antigen-presenting cells, such as the dendritic cell, could prove to be advantageous.

[0051]In this example, we describe the use of a charged and branched lipopeptide structure that can electrostatically bind protein antigens and deliver them to dendritic cells. The charge of the lipopeptide is mediated by the presence of four N-terminal arginine (R4) or glutamic acid residues (E4) imparting either an overall positive or negatively charge respectively to the delivery module. Hence R4 can be used to bind negatively charged prot...

Example

Example 2

[0061]In order to measure the ability of the branched R4(S2Pam2Cys) and the linear Pam2Cys-SK4 lipopeptide to form a complex with the model antigen ovalbumin (OVA), the light absorbances of solutions containing increasing amounts of each lipopeptide and OVA were determined by measuring the optical density at 450 nm (FIG. 3). The ability of each lipopeptide to allow complex formation with OVA would therefore indicate an association between the two compounds.

[0062]It was found that the addition of 5 nmoles of branched R4(S2Pam2Cys) to a solution containing 1 nmole of OVA resulted in the formation of complexes which appeared as a turbid or opalescent solution. Increasing the amount of branched lipopeptide resulted in an increase in optical density readings with the highest achieved when 20 to 40 nmoles of lipopeptide was used. In contrast, very little increase in optical density was observed when the linear Pam2Cys-SK4 lipopeptide was added at all concentrations investigated i...

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Abstract

The present invention provides an immunogenic composition comprising an antigen and a dendritic cell targeting component. A charged group is covalently attached to a dendritic cell ligand and is electrostatically associated with the dendritic cell targeting component.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]The present application is a continuation-in-part of International Patent Application No. PCT / AU2009 / 000446, filed Apr. 9, 2009, and is a continuation of International Patent Application No, PCT / AU2009 / 000469, filed Apr. 16, 2009, the disclosures of which are incorporated by reference herein in their entirety, including any figures, tables, or drawings.BACKGROUND OF INVENTION[0002]There is an ever increasing interest in the development of compositions which can be used to raise an immune response in humans and other animals in particular to protect against disease. In generating an immune response it is necessary for the antigen to encounter dendritic cells. Dendritic cells are immune cells and form part of the mammalian immune system. Their main function is to process antigen material and present it on the surface to other cells of the immune system, thus functioning as antigen-presenting cells.[0003]The targeting of antigens to dendriti...

Claims

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Application Information

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IPC IPC(8): A61K39/00C08B37/00C07C53/126C07K2/00A61P37/04
CPCA61K31/23A61K38/03A61K38/38A61K39/39A61K45/06A61K2039/6018A61K2300/00A61P37/04A61K2039/55516C07K2/00C07K14/77
Inventor JACKSON, DAVID CHARLESZENG, WEIGUANGCHUA, BRENDON YEW LOONG
Owner UNIVERSITY OF MELBOURNE
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