Composition and method for inhibiting, preventing, or ameliorating complications associated with ingestion of a medicinal, chemical, or biological substance or agent
a technology of biological substance and compound, applied in the direction of antinoxious agents, drug compositions, peptide/protein ingredients, etc., can solve the problems or in some instances preventing, death, injury, or other harm by producing diarrhea, so as to prevent or reduce the likelihood of, or prevent death, injury or other harm the effect and reducing the likelihood of reducing the likelihood o
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example 1
Establishment of STa Dose-Response Curve of STa to Establish Diarrheagenic Dose in a Population of Adult Mice
Diarrheal Response Assay
[0128]0.5 ml of 10% glucose solution with STa at different dilutions were administered to adult male Swiss Webster mice (each weighing 27-33 grams) by gavage. Mice were weighed prior to STa challenge. At each dilution of STa, 10 mice were utilized for establishing a statistical characterization of the diarrheal response (mean and standard deviation). After STa challenge, at approximately 90 minutes, the animals were sacrificed and the gut is removed and weighed. The remaining carcass is also weighed. The gut / body weight ratio is established as the in-vivo assay of quantifying the diarrheal response to exogenous STa.
[0129]Results are shown below:
Mouse GroupMean (Gut / BodyStandard(10 Mice in each Group)Weight) RatioDeviationControl (Saline).1084.0066 10 MU of STa.1066.0094100 MU of STa.1144.00651000 MU of STa .1339.0047***One MU is the amount of toxin whi...
example 2
Dose-Response Curve for Acetaminophen-Induced Hepatotoxicity
[0132]Data from several studies suggests that acetaminophen is responsible for 39 percent of all cases of acute liver failure in the United States. E.g., Lee, New England J. Med. (2003) 349: 474-485, Shankar et al., Toxicol. Sci. (2003) 73: 220-234. Acetaminophen-induced hepatotoxicity is also a well-established model of fulminant hepatic failure. E.g., Newsome et al., Liver Transplantation (2000) 6: 21-31.
Acetaminophen Administration
[0133]Food, but not water, will be removed 12 hours before acetaminophen administration. The method for acetaminophen administration will be as described by Walker et al, Lab Invest. (1980) 42:181-189. Briefly, acetaminophen will be dissolved in warm distilled water at various dilutions and given to mice by gavage at a volume of 0.5 ml. At each dilution of acetaminophen, 5 mice will be utilized for statistical characterization (mean and standard deviation) of physiologic responses to acetaminop...
example 3
Evaluation of Protective Drug Delivery Model to Prevent Acetaminophen-Induced Hepatic Failure in Adult Mice
[0138]In this section, a formulation of acetaminophen combined with STa will be evaluated for preventing hepatotoxicity due to acute acetaminophen overdosing. There will be three experimental groups and one control group. Group A will include mice administered a hepatotoxic dose of acetaminophen in a particulate (solid) form after a diarrheagenic dose of STa. Group B will include mice administered a hepatotoxic dose of acetaminophen dissolved in solution after a diarrheagenic dose of STa. Group C will include mice administered a therapeutic dose of acetaminophen in particulate (solid) form after a non-diarrheagenic dose of STa. The control group will consist of mice administered a hepatotoxic dose of acetaminophen in particulate (solid) form without any STa administration. Each experimental group and the control group will consist of 5 mice. Mice will be weighed at the beginni...
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