Human antibody against aggrecanase-type adamts species for therapeutics of aggrecanase-related diseases

Inactive Publication Date: 2016-10-20
GENEFRONTIER CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0022]According to the present invention, an anti-human aggrecanase antibody (particularly, anti-human ADAMTS4 antibody) useful for the prophylaxis or treatment of the progression of arthritis is provided.

Problems solved by technology

However, because there is little information about the biochemical character, expression patterns or gene promoter structures of mouse ADAMTS4 and ADAMTS5, the data from knockout mice must be interpreted carefully and should not be extrapolated to the human disease OA and RA [9, 10].

Method used

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  • Human antibody against aggrecanase-type adamts species for therapeutics of aggrecanase-related diseases
  • Human antibody against aggrecanase-type adamts species for therapeutics of aggrecanase-related diseases
  • Human antibody against aggrecanase-type adamts species for therapeutics of aggrecanase-related diseases

Examples

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examples

[0085]The present invention is explained in more detail in the following by referring to Examples, which are not to be construed as limitative. Various gene manipulations in the Examples followed the method described in Molecular cloning third. ed. (Cold Spring Harbor Lab. Press, 2001).

Materials and Methods

Phage Display Library Panning and Fab Generation

[0086]Production of monoclonal Fab antibodies specific to ADAMTS4 and ADAMTS5 were generated using the Human Combinatorial Antibody Library (HuCAL; MorphoSys AG, Martinried, Germany). Recombinant ADAMTS4 and ADAMTS5 (R&D Systems Inc., Minneapolis, Minn.) were biotinylated and incubated with HuCAL. Bound Fab expressing phages were enriched in three consecutive panning rounds. The pool of Fab genes was isolated from phagemids and inserted into Escherichia coli expression vectors that lead to functional periplasmic expression of Fab equipped with Strep-tag II. After transformation, individual colonies were picked up and grown in microti...

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Abstract

The present invention provides an antibody that specifically binds to human aggrecanase, and inhibits enzymatic activity of the human aggrecanase. In one embodiment, aggrecanase is ADAMTS4. In one embodiment, the antibody recognizes a particular epitope in human ADAMTS4, and inhibits not only aggrecanase activity of human ADAMTS4 but also aggrecanase activity of human ADAMTS5. In addition, the present invention also provides use of said antibody in the prophylaxis or treatment of the progression of arthritis.

Description

TECHNICAL FIELD[0001]The present invention relates to an anti-human aggrecanase antibody, and pharmaceutical use thereof.BACKGROUND ART[0002]Aggrecan degradation and subsequent digestion of collagen fibrils are the central pathway for the destruction of cartilage in human joint diseases including osteoarthritis (OA) and rheumatoid arthritis (RA). Collagen degradation is carried out principally by collagen-degrading matrix metalloproteinases (MMPs) such as MMP-1, MMP-8 and MMP-13 [1-3]. On the other hand, aggrecan-degrading metalloproteinases called aggrecanases are considered to play a key role in the aggrecan degradation [4, 5]. Aggrecanases belong to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) gene family, and ADAMTS1, 4, 5, 8, 9 and 15 are known to have aggrecanase activity [4, 6]. Recent studies using ADAMTS4 and ADAMTS5 knockout mice have indicated that ADAMTS5, but not ADAMTS4, plays an essential role in aggrecan degradation in mouse arthritides...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07K16/40
CPCC07K16/40C07K2317/565C07K2317/76C07K2317/34C07K2317/55C07K2317/21C07K2317/33A61P19/02A61P43/00
Inventor MIYAKOSHI, AKIRANAKAMURA, MIKIKOKOJOH, KANEHISAMOCHIZUKI, SATSUKIOKADA, YASUNORI
Owner GENEFRONTIER CORP
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