A Method for Mitigating Osteoporosis Using Metallic Gold Cluster Molecules
a cluster molecule and metal technology, applied in the direction of heavy metal active ingredients, drug compositions, pharmaceutical delivery mechanisms, etc., can solve the problems of osteoclast overabundance, affecting the effectiveness of osteoclasts, and more fragile bone structur
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example 1
[0050]For clarity reasons, the example in this application was conducted using a published peptide sequence (SEQ ID NO:1) Cys-Cys-Tyr-Gly-Gly-Pro-Lys-Lys-Lys-Arg-Lys-Pro-Gly. See Liu, R., et al, “The Au Clusters Induce Tumor Cell Apoptosis via Specifically Targeting Thioredoxin Reductase 1 (TrxR1) and Suppressing Its Activity”, Chem. Commun., 2014, 50, 10687-10690. The chemical reaction is as the following:
Peptide-TyrOH+OH−+HAuCl4→Peptide-Tyr=O+Au25(peptide)9 (I)
[0051]All chemicals were purchased from Sigma-Aldrich, unless otherwise indicated. Ultrapure water was used throughout the experiments. The peptides with 95% purity were chemically synthesized by a solid phase method (China Peptides Co. Ltd.). All glassware were washed with aqua regia, and then rinsed with ultrapure water and ethanol. An aqueous solution of HAuCl4 (25 mM, 80 μL) was slowly added to peptide solution (1.06 mM, 1880 μL) in a 5 mL vial under vigorous stirring at room temperature, then NaOH (0.5 M, 40 μL) was ad...
example 2
[0053]Another metallic gold-peptide cluster complex sample was made by using peptide (SEQ ID NO: 2) Glu-Cys-Gly (GSH) with the published procedure similar to the procedure in EXAMPLE 1 with the chemical reaction (II). See Luo, Z., et al, “From Aggregation-Induced Emission of Au(I)-Thiolate Complexes to Ultra bright Au(0)@Au(I)-Thiolate Core-Shell Clusters”, J. Am. Chem. Soc., 2012, 134, 16662-16670.
GSH+OH−+HAuCl4→Au25(SG)18+GS-SG (II)
[0054]FIG. 3C is the mass spectrometry of the generated gold-peptide cluster molecules showing a molecule formula of Au25(SG)18. FIGS. 3A and 3B show the absorbance and fluorescence emission spectra of the gold Au25(SG)18 cluster molecules. The absorbance and the maximum fluorescence emission peaks are located at 294 nm and 607 nm, respectively.
example 3
[0055]Another metallic gold-peptide cluster complex sample is made by using human serum album protein (HSA) with the following chemical reaction:
HSA-TyrOH+OH−+HAuCl4→HSA-Tyr=O+HSA-Au4000 (III)
[0056]All chemicals were purchased from Sigma-Aldrich, unless otherwise indicated. Ultrapure water was used throughout the experiments. All glassware were washed with aqua regia, and then rinsed with ultrapure water and ethanol. Aqueous HAuCl4 solution (5 mL, 10 mM, 37° C.) was added to HAS solution (5 mL, 5 mg / mL, 37° C.) under vigorous stirring. Two minutes later, NaOH solution (0.5 mL, 1 M) was introduced and the reaction was allowed to proceed under vigorous stirring at 37° C. for 12 hours. After the reaction, the sample was concentrated by a dialysis tube (MWCO: 100 kDa) to remove un-reacted free HSA, NaOH and HAuCl4. The obtained HSA-bound AuCs are suspended in the water and kept in dark at 4° C. UV-VIS spectrum (FIG. 4) of HSA-bound metallic gold cluster molecules shows the characterist...
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