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Screening methods using canine t2r receptors and pet food products and compositions identified using the same

a technology of canine t2r receptor and t2r receptor, which is applied in the direction of animal/human proteins, peptides, instruments, etc., can solve the problems of notorious finicky domestic animals, and achieve the effects of enhancing, increasing, decreasing, and enhancing, increasing, decreasing and/or modulating the activity and/or expression of a bitter taste receptor

Inactive Publication Date: 2020-09-17
MARS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach allows for the development of palatable pet food products by selecting raw materials and finished products that do not activate bitter receptors, and the creation of taste deterrents that effectively reduce palatability and ingestion of harmful substances by pets.

Problems solved by technology

Domestic animals are notoriously finicky in their food preferences, and often refuse to eat a pet food product that it has accepted over time or refuse to eat any more than a minimal amount of a pet food product.

Method used

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  • Screening methods using canine t2r receptors and pet food products and compositions identified using the same
  • Screening methods using canine t2r receptors and pet food products and compositions identified using the same
  • Screening methods using canine t2r receptors and pet food products and compositions identified using the same

Examples

Experimental program
Comparison scheme
Effect test

example 1

In Silico Model of Interactions Between Canine T2R Receptors and Putative Binding Compounds

[0167]The present example describes the computational modeling of canine bitter taste receptors (T2Rs) to identify putative bitter taste receptor modulators.

[0168]Homology models of canine T2R receptors were based on crystal structure of 3SN6 from Protein Data Bank (PDB). 3SN6 is the crystal structure of β2 adrenergic receptor from Group A GPCR with bound agonist (BI-167107 from Boehringer Ingelheim). (Rasmussen et al., Nature, 477: 549-555 (2011)). The models were built using the I-TASSER Suite of programs (Yang et al., Nat Methods, 12: 7-8 (2015)) and Modeller (Eswar et al., Curr Protoc Bioinformatics, 15: 5.6.1-5.6.30 (2006)), which is part of the DiscoveryStudio (DS) suite of programs from Accelrys (DiscoveryStudio (DS) is suite of interactive modeling and simulation programs from the Accelrys corporation).

[0169]The bitter compounds were docked into the active site of canine bitter recepto...

example 2

Identification of Canine Bitter Receptor (T2R) Modulators Using in Vitro Assays.

[0171]The present example describes an in vitro assay for identifying compounds that modulate the activation of the canine bitter taste receptor (T2R).

[0172]Compounds identified by in silico modeling with a bitter taste receptor, as detailed above in Example 1, as putative bitter taste receptor modulators will be selected for further testing in vitro. In vitro functional characterization of the selected modulators will be used to evaluate the effectiveness of a putative modulator compound in activating or inhibiting the bitter taste receptor.

[0173]HEK293 cells (or other suitable expression system) that stably or transiently express a canine bitter taste receptor (e.g., canine T2R1, T2R2, T2R3, T2R4, T2R5, T2R7, T2R10, T2R12, T2R38, T2R39, T2R40, T2R41, T2R42, T2R43, T2R62, or T2R67) will be exposed to putative compounds to modulate the activity and / or expression of the bitter taste receptor.

[0174]An exem...

example 3

Identification of Canine Bitter Receptor (T2R) Modulators Using in Vitro Assays.

[0177]The present example describes an in vitro assay for identifying compounds that modulate the activation of the canine bitter taste receptor (T2R) by a T2R ligand.

[0178]Compounds identified by in silico modeling with a bitter taste receptor, as detailed above in Example 1, as putative bitter taste receptor modulators will be selected for further testing in vitro. In vitro functional characterization of the selected modulators will be used to evaluate the effectiveness of a putative modulator compound in modulating the activation of the bitter taste receptor by a bitter taste receptor ligand.

[0179]HEK293 cells (or other suitable expression system) that stably or transiently express a canine bitter taste receptor (e.g., canine T2R1, T2R2, T2R3, T2R4, T2R5, T2R7, T2R10, T2R12, T2R38, T2R39, T2R40, T2R41, T2R42, T2R43, T2R62, or T2R67) will be exposed to putative modulator compounds and a bitter taste re...

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Abstract

The presently disclosed subject matter relates to methods of screening raw materials and pet food products to manufacture a palatable pet food product. The presently disclosed subject matter also relates to methods for identifying compounds that modulate the activity and / or expression of a bitter taste receptor.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application is a Continuation of U.S. patent application Ser. No. 15 / 746,658, filed on Jan. 22, 2018, which claims priority to International Patent Application No. PCT / US2016 / 044540, filed on Jul. 28, 2016, which claims priority to U.S. Provisional Application Ser. No. 62 / 197,983, filed on Jul. 28, 2015, the content of each are incorporated by reference in their entireties, and to which priority is claimed.SEQUENCE LISTING[0002]The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on May 27, 2020, is named 0692690239CONSEQ.txt and is 65,750 bytes in size.FIELD[0003]The presently disclosed subject matter relates to the use of canine T2R bitter taste receptors (cT2Rs) for the identification of T2R modulators. The presently disclosed subject matter further relates to the use of canine T2R bitter taste re...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/50C07K14/72A23L27/00A23K20/147A23K50/40G01N33/566
CPCA23K20/147G01N33/566G01N33/502A23K50/40G01N33/5008G01N2333/726C07K14/723A23L27/84
Inventor GIBBS, MATTHEW RONALDDESFORGES, NEIL GEORGETAYLOR, ANDREW JOHNMCGRANE, SCOTT JOSEPHKLEBANSKY, BORISFINE, RICHARD MASTEN
Owner MARS INC
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