The present invention provides a
recombinant Escherichia coli for synthesizing anisic acid from scratch, wherein at least one of the following improvements is made to the starting strain of
Escherichia coli: (1) overexpressing O-
methyltransferase PSA-OMT1 from
Pleurotus sapidus; overexpressing chorismate
lyase UbiC from E. coli MG1655 or
Providencia rustigianii; (2) overexpressing a
feedback inhibition mutant AroG of 3-deoxy-D-arabino-
heptanoic acid 7-
phosphate (DAHP) synthase from E. coli MG1655 on the basis of (1); D146N (3) Based on (2), the
enzyme PpsA derived from phosphoenolpyruvate (PEP) synthase was overexpressed, and the pykF
gene encoding
pyruvate kinase I was knocked out. Thus, the recombinant E. coli of the present invention can achieve accumulation of anisic acid in the
fermentation broth during the
fermentation process, and the maximum anisic acid yield of the recombinant E. coli in a
shake flask can reach 284.9 mg / L, laying the foundation for the subsequent construction of high-yield anisic acid engineered
bacteria.