The invention discloses a constant temperature control method. The method comprises the following steps that a temperature error, an error accumulation and a temperature control output are calculated;when the temperature control output is greater than a first preset threshold, the heater power is adjusted according to the first preset threshold, and a preset constant temperature and a detection voltage are obtained; the temperature error is calculated according to the preset constant temperature and the detection voltage, and whether the temperature error is less than zero or not is judged, if yes, the error accumulation is calculated, and if not, the error accumulation is not calculated; and when the temperature control output is smaller than a second preset threshold, the heater power is adjusted according to the second preset threshold, and the preset constant temperature and the detection voltage are obtained; and the temperature error is calculated according to the preset constant temperature and the detection voltage, whether the temperature error is greater than zero or not is judged, if yes, the error accumulation is calculated, and if not, the error accumulation is not calculated. Correspondingly, the invention further discloses a constant temperature control system. By adopting the constant temperature control method and system, timely adjustment can be carried out according to the real-time water temperature, and the stability of temperature regulation is improved.