The invention provides a separation and culture method of renal
podocyte of a mouse. The separation and culture method comprises the following steps of (a), intraperitoneally injecting 2% pentobarbital
sodium to execute the mouse, sterilizing the mouse with
alcohol, taking out
kidney tissue of the mouse, putting the
kidney tissue into a precooled sterile PBS (
Phosphate Buffer solution) containing double antibodies, soaking and washing the
kidney tissue, and peeling and removing a renal
capsule; (b), mechanically dissociating the kidney tissue, then digesting the kidney tissue by a preheated mixed
enzyme solution, and terminating
digestion by a mixed complete culture solution; (c), obtaining the renal
podocyte by adopting a difference sieving method, inoculating the
podocyte in a
treated cell bottle, and culturing the podocyte; (d), digesting a
cell, which is cultured for 3d, through pancreatin, then filtering the
cell once again by a
sieve mesh, centrifuging filtrate to remove supernatant fluid, and resuspending and inoculating an obtained mixture in the
treated cell culture flask; (e), identifying the renal podocyte by
cell immunofluorescence. The separation and culture method of the renal podocyte of the mouse, which is provided by the invention, is good in
repeatability, and is simple and convenient to operate; moreover, the high-yield and high-purity renal podocyte of the mouse can be obtained.