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Composition of mycobacterium polysaccharide and its preparation

A technology of mycobacterial polysaccharides and mycobacteria, which is applied in the direction of bacteria, drug combinations, allergic diseases, etc., can solve the problems of dangerous production process, long cultivation cycle, long production cycle, etc., and achieve short production cycle and wide range of toxic and side effects , the effect of production safety

Inactive Publication Date: 2008-01-02
北京首医科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, S.S.M is a polysaccharide extracted from Mycobacterium tuberculosis. Because Mycobacterium tuberculosis is highly infectious to humans, its production process is dangerous; long
In addition, it is reported that 4.7% of patients with S.S.M have local allergic reactions after injection, indicating that the preparation has certain toxic and side effects, which limits its application to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Mycobacterium herbaceous was cultured and maintained in Roche's egg slant medium. Use Sutong medium for expansion culture, 110-120ml per bottle, after 2 weeks of cultivation in a greenhouse at 35°C-37°C, the culture bottle shows that yellow to orange pellicles grow. The liquid under the bacterial film in the culture bottle should be clear and transparent, there should be no growth of gray-green or gray-black pollutants, and there should be no odor or peculiar smell. The culture must be inspected bottle by bottle to ensure that it is not contaminated and abnormal before it can be used for the extraction of polysaccharides.

[0041] Concentrate the above cultures in a large glass beaker, heat and boil in boiling water for 2 hours, and then centrifuge at 4000 rpm for 20 minutes after cooling to completely precipitate the bacterial sediment and the supernatant is clear and transparent. Collect the supernatant, add glacial acetic acid, the amount of glacial acetic acid acco...

Embodiment 2

[0046] A large number of experimental studies have shown that the present invention has the effects of widely improving the body's immune function and increasing white blood cells, prepared in Example 1. Human application without any side effects.

[0047]In order to test the effect of the compound of the present invention on enhancing the phagocytosis and killing activity of mouse macrophages, an experiment on the effect of the compound of the present invention on the phagocytosis and killing activities of mouse macrophages was carried out. Experiments were performed using mice of 20-25 g. The animals were randomly divided into two groups with 10 animals in each group. Inject the compound of the present invention 0.5ml (50μg / ml) in the experimental group mice intraperitoneally, and inject 0.5ml normal saline in the control group intraperitoneally, after 16h, wash the mice with 3ml of RPMI1640 solution (please indicate the source) (pH7.4) For the peritoneal cavity, place the...

Embodiment 3

[0060] Eliminate the reduction of leukocytes in mice caused by immunosuppressant (cyclophosphamide) and promote its recovery

[0061] Animals: LACA mice, male, 18-22 g, were purchased from the Animal Center of Beijing Medical University.

[0062] Drug: MPS is a water injection in ampoules, divided into 4 specifications: 60μg / ml, 80μg / ml, 100μg / ml and 120μg / ml. Wheel blood (GRAN) is an ampoule injection of 75 P8, a product of Japan Kirin Brewery Co., Ltd., batch number: 483A. Cydophosphamide (Cydophosphamide, CP) is a 20mg powder per bottle, a product of Shanghai No. 12 Pharmaceutical Factory, batch number: 920317.

[0063] Experiment grouping and administration: Each batch of experiments was divided into 7 groups, with 10 mice in each group. The normal control group was healthy LACA mice, which were injected with normal saline instead of administration; the cyclophosphamide group was given CP at 120 mg / kg, and administered intraperitoneally once a day on the 6th and 7th day;...

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PUM

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Abstract

A polyopse composition composed of arabinose, mannose, glucose and galactose for treating leukocytopenia of cancer patient, chronic hepatitis and multiple immunopathies is extracted from the nonpathogenic mycobacteria.

Description

technical field [0001] The invention relates to an immunotherapeutic drug effective for treating various diseases. Specifically, the present invention relates to a pure polysaccharide complex extracted from whole non-pathogenic mycobacteria and a preparation method. Background technique [0002] Current research shows that polysaccharides derived from some fungi, bacteria and traditional Chinese medicine can significantly enhance the immune function of the body. Among them, more researches are related to the immune function and application of mycobacteria. Studies on the immune function of mycobacteria can be roughly summarized into two aspects. On the one hand, it is used as an immune adjuvant, such as Bacillus Calmette-Guerin (BCG) prepared by Mycobacterium bovis and muramyl dipeptide (MDP) prepared by extracting from the cell wall of mycobacteria. Such substances have been used for a long time. They belong to non-specific immune enhancers. In addition to being used in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P37/04C12N1/20C08B37/00
Inventor 陈仁郭建强潘燕春郜文
Owner 北京首医科技有限公司
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