Method for determining hypocrellin A content by high efficiency liquid phase chromatographic method

A kind of high performance liquid chromatography, the technology of bamboo rhodopsin A, applied in the field of chromatographic detection, can solve the problems such as difficulty in the chromatographic analysis method of rhododendron 1, the separation effect and recovery rate cannot be achieved, and there is no rhodopsin A. , to achieve the effect of simple method, high sensitivity and good linear relationship

Inactive Publication Date: 2008-05-14
NANJING NORMAL UNIVERSITY
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Problems solved by technology

[0005] The reason why there are no research results on the chromatography of hypocrellin A is that it is quite difficult to find a chromatographic analysis method suitable for hypocrellin A
A large number of experiments have shown that Hypocretin A presents flat chromatographic peaks with tailing for several minutes on general C18, C8, and ion-exchange chromatographic columns, changing the elution strength of the mobile phase, pH value, buffer Salt concentration, etc., cannot be optimized; the chromatographic peak of hypocrellin A is not only poor in peak shape, but also has a very low number of theoretical plates. In the analysis of the extract of bamboo red fungus, the separation effect and recovery rate cannot be achieved.

Method used

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  • Method for determining hypocrellin A content by high efficiency liquid phase chromatographic method
  • Method for determining hypocrellin A content by high efficiency liquid phase chromatographic method
  • Method for determining hypocrellin A content by high efficiency liquid phase chromatographic method

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Embodiment 1

[0024] Take the bamboo red fungus sample collected from Pujiang County, Yunnan, dry and pulverize, accurately weigh about 1g, add 250ml of methanol, weigh, and reflux in a water bath at 70°C for 50min under dark conditions. After cooling, add methanol to make up for the lost mass, shake well and filter. After the filtrate was passed through a 0.45 μm microporous membrane, the sample was injected, and the content of hypocrellin A in it was analyzed, and the content of hypocrellin A in it was obtained to be 28.0 mg / g respectively.

Embodiment 2

[0026] Take the bamboo red fungus sample collected from Ninglang County, Yunnan, dry and pulverize, accurately weigh about 1g, add 250ml of methanol, weigh, and reflux in a water bath at 70°C for 50min under dark conditions. After cooling, add methanol to make up for the lost mass, shake well and filter. After the filtrate was passed through a 0.45 μm microporous membrane, the sample was injected, and the content of hypocrellin A was analyzed, and the content of hypocrellin A in it was respectively 27.2 mg / g.

[0027] Working principle and working process

[0028] The mobile phase methanol and water carry the substances to be tested such as hypocrellin A into the chromatographic column, and the ZorbaxExtend-C18 chromatographic column separates hypocrellin A from other impurities, so that they arrive at the fluorescence detector in turn to be detected and identified, and the detector gives The response values ​​of different components are displayed, corresponding to the amount...

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Abstract

The high efficiency liquid phase chromatographic process of determining hypocrellin A content includes the following steps: a. selecting Zorbax Extend-C18 column; b. adopting mixture of methanol and water in the volume ratio of 83 to 17 as chromatographic flow phase; and c. selecting conditions of flow rate 1 ml / min, fluorescent detector exciting wavelength 410 nm, emitting detection wavelength 610 nm and sample volume 10 microliter. The present invention provides proper chromatographic analysis method of hypocrellin A.

Description

technical field [0001] The invention relates to a chromatographic detection method, in particular to a method for determining the content of hypocrellin A by high performance liquid chromatography. Background technique [0002] Hypocrellin is a new type of phototherapy drug discovered and applied clinically for the first time in my country. It has been clinically proven that it has obvious photosensitive therapeutic effect on certain stubborn skin diseases, including hypertrophic scars, skin amyloid lichen, psoriasis, and vitiligo. After more than ten years of extensive and systematic research on the structure, photochemistry, photophysics, photobiology, and pharmacology of hypocrellin, researchers have found that compared with other types of photosensitizers, hypocrellin acts as A new type of photosensitizer has the advantages of easy purification, good chemical modification, high triplet quantum yield, high singlet oxygen quantum yield, high phototoxicity, and fast excret...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/74G01N30/88
Inventor 周家宏顾晓天冯玉英魏少华
Owner NANJING NORMAL UNIVERSITY
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