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Rana grahami propyl sperm peptide, gene and variant and its pharmaceutical use

A technology of variants and protidines, applied in the field of biomedicine, achieves obvious effects, simple structure, and convenient artificial synthesis

Inactive Publication Date: 2008-05-21
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports about the research on the active substances of the fingerless frog skin

Method used

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  • Rana grahami propyl sperm peptide, gene and variant and its pharmaceutical use
  • Rana grahami propyl sperm peptide, gene and variant and its pharmaceutical use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0038] Cloning of the arginine gene of the fingerless frog:

[0039] I. Extraction of total RNA from the skin of the fingerless frog:

[0040] A. The live fingerless frog is cleaned with water, put into liquid nitrogen and quick-frozen for 4 hours, get the skin tissue, weigh, get 300mg skin tissue, add 10ml total RNA extraction buffer (Trizol solution, U.S. GIBCOBRL company product), Homogenize for 30 minutes in a 20ml glass homogenizer.

[0041] B. Add an equal volume of phenol / chloroform solution, mix vigorously, leave at room temperature for 10 minutes, centrifuge at 12,000 rpm for 10 minutes at 4°C, and discard the precipitate.

[0042] C. Add an equal volume of isopropanol to the supernatant, leave it at room temperature for 10 minutes, centrifuge at 12,000 rpm for 10 minutes at 4°C, wash the precipitate once ...

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Abstract

The invention relates to non finger odor frog third refined peptide, gene and the variant and the application in medicine. It is an annular polypeptide, and the molecular weight is 2188.68 dalton, isoelectric point is 10.07, the full sequence is NH2- AALKGCWTKSIPPKPCFGKR-COOH, the sixth cysteine and the sixteenth cysteine form intramolecularly disulfide bond. The coding gene is formed by 304 nucleotides. And the coding mature sections are the 130th to the 189th nucleotides. It could be used to cure tumor, gastritis, and pancreatitis, and has simple sequence.

Description

Technical field: [0001] The invention provides a protidine, gene and variant of Rana grahami, belonging to the technical field of biomedicine. Background technique: [0002] The most basic function of serine protease inhibitor (serpin) is to prevent proteolysis and regulate the hydrolysis balance of serine protease. Through the regulation of serine proteases, serine protease inhibitors have important effects on many important physiological and biochemical functions in organisms. The following 14 physiological and biochemical functions are regulated by serine protease inhibitors: blood coagulation, complement formation, fibrinolysis, protein folding, cell migration, cell differentiation, cell matrix remodeling, hormone formation and transport, intracellular proteolysis, blood pressure regulation, Tumor suppression and formation of viral or parasitic pathogenicity. Because so many physiological functions are regulated by it, serine protease inhibitors have become the focus o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435A61K38/57A61P35/00A61P37/02C12N15/12A61K48/00
Inventor 赖仞李建许李东升徐学清韩耀平
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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