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Method for preparing general saponin of golden Chinese gentian and application

A technology of gentian grass and total saponins is applied in the application field of anti-digestive tract ulcer medicine, can solve the problems of unsuitability for industrialized production, low yield, environmental pollution and the like, achieves clear anti-digestive tract ulcer activity and is suitable for industrialization Production, the effect of reducing production costs

Inactive Publication Date: 2008-12-31
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, as mentioned above, need to use silica gel to process the ethanol extract of Gentiana gentiana in step 2 of this preparation method, because silica gel cannot be recovered after use, so it can cause serious pollution to the environment, and in this step There are many kinds of organic solvents used, so the time required for processing is long and the operation process is complicated, which will not only increase the production cost, but also is not suitable for industrial production
Especially in the process of using silica gel to process the ethanol extract of Gentiana gentiana, it will cause dead adsorption, resulting in a lower yield of less than 1.2%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Extract 17 kg of the crushed whole herb of Gentiana radix with 95% ethanol under reflux for 2 times, each time for 2 hours, after filtering, extract the filter residue with 60% ethanol under reflux for 2 hours and filter, combine the two filtrates, and then depressurize Concentrate until ethanol is completely evaporated. Dilute the above concentrated solution to 11 liters with distilled water, and the concentration at this time is 17g / 100ml, then extract 4 times with an equal amount of ethyl acetate in a separating funnel to remove impurities, and evaporate the water layer under reduced pressure until the ethyl acetate is completely Volatilize, then extract 7 times with an equal amount of n-butanol in a separatory funnel, and finally concentrate the combined n-butanol extracts under reduced pressure to obtain 602 g of crude saponin. The above crude saponin was dissolved in water and subjected to macroporous resin D101 column chromatography (1610g, chromatographic column...

Embodiment 2

[0025] 62g of dried and pulverized Golden Gentiana was extracted twice with 94% ethanol under reflux, each time for 2 hours; after filtration, the filter residue was extracted under reflux with 61% ethanol for 2 hours and filtered, the two filtrates were combined, and then Concentrate under reduced pressure until ethanol evaporates completely. Dilute the above concentrated solution to 100ml with distilled water, the concentration at this time is 11g / 100ml, then extract 4 times with an equal amount of ethyl acetate in a separating funnel to remove impurities, and concentrate the water layer under reduced pressure until the ethyl acetate is completely volatilized , and then extracted 7 times with an equal amount of n-butanol in a separatory funnel, and finally concentrated the combined n-butanol extracts under reduced pressure to obtain 3.5 g of crude saponin. Dissolve the above crude saponin in water and perform column chromatography on macroporous resin D101, then carry out gr...

Embodiment 3

[0027]1 kg of dried and pulverized Gentiana radix was extracted twice with 98% ethanol under reflux, each time for 2 hours, after filtering, the filter residue was extracted with 63% ethanol under reflux for 2 hours and filtered, the two filtrates were combined, and then Concentrate under reduced pressure until ethanol evaporates completely. Dilute the above concentrated solution to 1000ml with distilled water, and the concentration is 14g / 100ml at this time, then extract 4 times with an equal amount of ethyl acetate in a separating funnel to remove impurities, and concentrate the water layer under reduced pressure until the ethyl acetate is completely volatilized , and then extracted 7 times with an equal amount of n-butanol in a separating funnel, and finally concentrated the combined n-butanol extracts under reduced pressure to obtain 45.9 g of crude saponin. Dissolve the above crude saponin in water and perform column chromatography on macroporous resin D101, then carry ou...

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Abstract

A process for preparing the general saponin of golden gentian used for treating the ulcer in digestive tract includes such steps as extracting in alcohol and then in ethylacetate and n-butanol, vacuum concentrating chromatography by macroreticular resin, gradient eluting, and vacuum concentrating.

Description

technical field [0001] The present invention relates to a preparation method and application thereof, in particular to a preparation method of total gentian saponins with low cost, little environmental pollution and suitable for industrial production and its application in anti-digestive tract ulcer drugs in the application. Background technique [0002] Liquorgrass is a plant belonging to the aster family of Compositae. There are 80-100 species in the world, mainly distributed in the tropical and subtropical regions of the eastern and western hemispheres. At present, there are about 10 species in our country, such as golden gentian grass, Sumen baijiu grass, flying cape, fragrant silk grass and small baijiu grass, etc., most of which are produced in the south and southwest regions. Among them, Gentiana is the dried whole herb of Artemisia blinii Lévl. in the genus of Baijiu. Experiments have proved that Gentiana contains diterpenoids, flavonoids, sesquiterpenoids, triterpe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/28A61P1/04
Inventor 苏艳芳杨凤英陈磊吕敏邸利芝
Owner TIANJIN UNIV
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