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Process for preparing starch-base immobilized enzyme vector

An immobilized enzyme carrier, starch-based technology, applied in the direction of immobilization on/in organic carrier, fermentation, etc., can solve the problems of restricting the application of starch, achieve important social and economic benefits, enhance adsorption capacity, immobilize The effect that the ability of the enzyme improves

Inactive Publication Date: 2009-02-11
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Starch can immobilize a variety of enzymes through adsorption, but the application of starch as an enzyme immobilization carrier is limited due to defects in specific surface area and other aspects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The first step is to adjust the concentration of 100 grams of potato starch to 82% by water, seal it in a container, react at a temperature of 120° C. for 1 hour, and cool to room temperature.

[0021] The second step is to adjust the concentration of starch milk to 15% mass with phosphate buffer solution, the pH value is 5.0, and the temperature is 45°C. Add 600 μ / g dry starch α-amylase and 50 μ / g dry starch glucoamylase, and react for 4 hours .

[0022] The third step is to add the enzyme-treated starch into 0.4mol / L sodium periodate solution, the molar ratio of sodium periodate to dry starch is 1.1:1, the reaction temperature is 30°C, the pH is 4.5, and the reaction is stirred for 5 hours .

[0023] The fourth step is drying and crushing to obtain a white powder product. By immobilizing lipase, the measurement results show that the starch carrier protein load is 45.6mg / g dry starch, and the activity recovery rate is 61.3%. After the starch is immobilized with lipase...

Embodiment 2

[0025] The first step adjusts the concentration of 100 grams of tapioca starch with water to be 70% by weight, seals it in a container, reacts at a temperature of 100°C for 24 hours, and cools to room temperature.

[0026] The second step is to adjust the concentration of starch milk to 20% mass with phosphate buffer solution, the pH value is 5.5, and the temperature is 55°C. Add 450μ / g dry starch α-amylase and 30μ / g dry starch glucoamylase, and react for 30 hours .

[0027] The third step is to add the enzyme-treated starch into 0.7mol / L sodium periodate solution, the molar ratio of sodium periodate to dry starch is 1.2:1, the reaction temperature is 50°C, the pH is 3.0, and the reaction is stirred for 2 hours .

[0028] The fourth step is drying and crushing to obtain a white powder product. Through the immobilization of lipase, the measurement results show that the starch carrier protein load is 53.2mg / g dry starch, and the activity recovery rate is 65.8%. After the starch...

Embodiment 3

[0030] The first step is to adjust the concentration of 100 grams of corn starch to 75% by water, seal it in a container, react at a temperature of 80° C. for 10 hours, and cool to room temperature.

[0031] The second step is to use phosphate buffer to adjust the concentration of starch milk to 45% by mass, the pH value is 5.5, and the temperature is 60°C. Add 500 μ / g of dry starch α-amylase, react for 4 hours, inactivate the enzyme, and adjust the pH value to 6.0 , the temperature is 65°C, add 40μ / g dry starch glucoamylase, and react for 10 hours.

[0032] The third step is to add the enzyme-treated starch into 0.5mol / L sodium periodate solution, the molar ratio of sodium periodate to dry starch is 1.1:1, the reaction temperature is 40°C, the pH is 3.0, and the reaction is stirred for 3 hours .

[0033] The fourth step is drying and crushing to obtain a white powder product. By immobilizing lipase, the measurement results show that the starch carrier protein load is 60.2mg / ...

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Abstract

The invention relates to the preparation method of starch-based solidified enzyme carrier. The method is to adjusting starch raw material with water to 70-82wt.%, placing into a closed container, treating at 80-120DEG C for 1-24h, cooling to room temperature, preparing to 15-45wt.% starch slurry with phosphate buffer, adjusting pH to 4.0-6.5, adding starch enzyme 30-600 mu / g of dry starch, carrying out a reaction at 45-65DEG C for 4-30h, adding into 0.4-0.7mol / L sodium metaperiodate solution at a sodium metaperiodate / dry starch mol. ratio of (1.1-1.4):1 at 30-50DEG C, and pH 3.0-4.5, stirring for 2-5, drying, and crushing to obtain the final product. The starch carrier can physically absorb, and chemically crosslink and solidify various enzymes, and has the advantages of high activity, multiple reutilization.

Description

technical field [0001] The invention relates to a production method of modified starch, in particular to a preparation method of a starch-based immobilized enzyme carrier utilizing biotechnology and chemical methods. Background technique [0002] Enzymes are a class of biological catalysts. Since enzymes are composed of proteins, their higher order structures are very sensitive to their environment. Under normal circumstances, it is not stable enough to heat, strong acid, strong alkali, organic solvent, etc., and it is easy to be inactivated in the reaction, and the enzyme often contains impurity proteins and colored substances, which makes it difficult to separate and purify the product, and limits the wide range of enzymatic reactions. application. Therefore, the enzyme immobilization technology developed since the 1960s not only overcomes the above shortcomings, but also maintains the unique catalytic activity of enzymes to a certain extent, thus becoming one of the mos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/00C12N11/10
Inventor 罗志刚罗发兴扶雄何小维
Owner SOUTH CHINA UNIV OF TECH
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