Method for producing 1,3-propylene from microorganism using glycerol anaerobic fermentation

An anaerobic fermentation and propylene glycol technology, applied in 1 field, can solve the problems of low fermentation conversion rate, achieve the effects of reducing production cost, improving substrate conversion rate, and shortening fermentation time

Active Publication Date: 2009-03-11
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

[0008] Aiming at the low conversion rate of glycerol anaerobic fermentation by a single bacterium, the present invention provides an improved method for synthesizing 1,3-propanediol by glycerol fermentation, that is, adding an appropriate amount of organic intermediate metabolism in the fermentation medium or anaerobic fermentation process Molecule, strengthen the subsequent metabolic process of pyruvate in the oxidative metabolic branch, so that the subsequent metabolic flow of pyruvate is mainly carried out towards the TCA cycle, so as to provide NADH required for the formation of 1,3-propanediol in the glycerol reduction branch, Weaken the tendency of bacteria to produce more metabolic streams such as acetic acid, lactic acid, ethanol, and methanol small molecule by-products under anaerobic conditions, improve the metabolic process of bacteria, increase the mutual coupling between growth and production of bacteria, and improve fermentation efficiency

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  • Method for producing 1,3-propylene from microorganism using glycerol anaerobic fermentation

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Embodiment 1

[0023] The bacterial classification used in the embodiment of the present invention is Klebsiella pneumoniae (Klebsiella pneumoniae), comes from the patent bacterial classification of Sinopec Fushun Petrochemical Research Institute, and the bacterial classification is in China General Microorganism Culture Collection Center (CGMCC), and the bacterial classification preservation number : 0798.

[0024] There are two types of medium: seed medium and fermentation medium:

[0025] Seed medium composition: (1L)

[0026] K 2 HPO 4: 34g KH 2 PO 4 : 13g

[0027] (NH) 4 SO 4 : 6g MgSO 4 ·7H 2 O: 0.2g

[0028] CaCl 2 ·H 2 O: 0.02g CaCO 3 : 2.0g

[0029] Yeast paste: 1.0g Glycerin: 60g

[0030] Fe 2+ Solution: 2.0mL Trace element solution I: 1.0mL

[0031] where Fe 2+ Solution: (FeSO 4 5g / L HCl (37%) 4.0mL)

[0032] Composition of trace element solution I (mg / L):

[0033] MgSO 4 4H 2 O: 100mg / L ZnCl 2 : 70mg / L

[0034] Na 2 MoO 4 2H 2 O: 35mg / LH 3 BO 3 : 60m...

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Abstract

This invention provides a promoting cell synthesizing trimethylene glycol method by adding organic intermediary metabolism molecule, and it belongs to trimethylene glycol biosynthesize technology field. Aim at the problem of low transformation efficiency of single cell using glycerine anaerobic fermentation in present technology, this invention realizes ideal flux distribution of substrate and product during the process of trimethylene glycol fermentation, which makes high effect trimethylene glycol fermentation into reality. The invention breaks through the bottleneck of low trimethylene glycol transformation efficiency using glycerine biosynthesis, which improves the transformation efficiency of substrate, shortens fermenting time and cuts down the cost.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for bioconverting fermentable glycerin carbon source into 1,3-propanediol by using a single microorganism. Background technique [0002] As we all know, 1,3-propanediol is an important chemical raw material and pharmaceutical intermediate, and it is a monomer with potential application value in the production of polyester fiber and in the manufacture of polyurethane and cyclic compounds. [0003] At present, chemical synthesis is mainly used in industry to produce 1,3-propanediol. It is known that there are many kinds of chemical ways to produce 1,3-propanediol, but there are many by-products in the chemical method, the separation and purification of the product are difficult, and the production cost is correspondingly high. In contrast, the production of 1,3-propanediol by biotransformation has attracted more and more attention because of its characteristics of ut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12R1/22
Inventor 王领民金平佟明友
Owner CHINA PETROLEUM & CHEM CORP
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