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Process for producing 1,3-propylene glycol by microbe fermentation

A technology for microbial fermentation and propylene glycol, applied in 1 field, can solve problems such as low fermentation conversion rate, and achieve the effects of reducing production cost, shortening fermentation time, and improving substrate conversion rate

Active Publication Date: 2011-08-24
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Aiming at the low conversion rate of glycerol anaerobic fermentation by a single bacterium, the present invention provides an improved method for synthesizing 1,3-propanediol by glycerol fermentation, which can not only effectively control the pH value of the fermentation process, but also efficiently reduce the acetic acid in the system and the accumulation of lactic acid byproducts, strengthen the subsequent metabolic process of pyruvate in the oxidative metabolic branch, so that the subsequent metabolic flow of pyruvate is mainly carried out towards the TCA cycle, so that when it is supplied to the glycerol reduction branch to form 1,3-propanediol While NADH is needed, it weakens the inhibitory effect of bacteria on the metabolic branch of acetic acid and lactic acid by-products under anaerobic conditions, improves the metabolic process of bacteria, increases the mutual coupling between growth and production of bacteria, and improves fermentation efficiency

Method used

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Embodiment 1

[0022] (1) The bacterial classification used in the embodiment of the present invention is Klebsiella pneumoniae (Klebsiella pneumoniae), comes from the patent bacterial classification of Sinopec Fushun Petrochemical Research Institute, and the bacterial classification is preserved in China General Microorganism Culture Collection Center (CGMCC), Culture preservation number: 0798.

[0023] (2) The medium is divided into two types: seed medium and fermentation medium:

[0024] Seed medium: (1L)

[0025] K 2 HPO 4 : 34g KH 2 PO 4 : 13g

[0026] (NH 4 ) 2 SO 4 : 6g MgSO 4 ·7H 2 O: 0.2g

[0027] CaCl 2 ·H 2 O: 0.02g CaCO 3 : 2.0g

[0028] Yeast extract: 1.0g Glycerol: 60g

[0029] Fe 2+solution: 2.0mL Trace element solution I: 1.0mL

[0030] where Fe 2+ solution (1L): FeSO 4 5g / L HCl (37%) 4.0mL

[0031] Composition of Trace element solution I(1L)

[0032] MgSO 4 4H 2 O: 100mg ZnCl 2 : 70mg

[0033] Na 2 MoO 4 2H 2 O: 35 mg H 2 BO 3 : 60mg

[0034] ...

Embodiment 2-12

[0054] They are the fermentation experiments carried out by using different basic calcium salts at different concentrations to implement online control of the pH value of the system.

Embodiment 13-15

[0061] In the case of using alkaline calcium salts to regulate the pH value of the fermentation system, the fermentation broth is directly subjected to ultrafiltration and nanofiltration to remove salt. The ultrafiltration uses an organic membrane with a molecular weight cut-off of 30,000, and the nanofiltration uses a roll-type membrane with a molecular weight cut-off of 300. The effect of sterilization, protein removal and desalination can be realized at the same time, and the conductivity of the obtained treatment solution is shown in Table 2.

[0062] As can be seen from the results in Table 2, the use of alkaline calcium salts to adjust the pH value of the fermentation process system, when the fermented liquid obtained is processed, the key desalination step is not satisfactory when the traditional method is used for separation, and the use of this method The effect of the ultrafiltration and nanofiltration process provided by the invention is ideal, and the operation step...

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Abstract

The invention provides a method for improving the production of 1, 3-propylene glycol through glycerol anaerobic fermentation. The method improves the metabolism of glycerol between a reduction branch and an oxidation branch so as to be propitious to thalli growth and product generation; meanwhile, the method not only can increase the concentration of 1, 3-propylene glycol in fermentation broth, but also can increase the molar transformation efficiency of glycerol; moreover, an ultrafiltration and nanofiltration desalinization method provided by the method has simple operation, low product loss rate and ideal effects.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for bioconverting fermentable glycerin carbon source into 1,3-propanediol by using a single microorganism. Background technique [0002] 1,3-propanediol (1,3-propanediol, referred to as 1,3-PDO) is an important chemical and pharmaceutical intermediate raw material. A monomer with potential application value. [0003] At present, the industry mainly adopts chemical synthesis to produce 1,3-propanediol. It is known that there are many kinds of chemical ways to produce 1,3-propanediol, but there are many by-products in the chemical method, the separation and purification of the product are difficult, and the production cost is correspondingly high. In contrast, the production of 1,3-propanediol by biotransformation has attracted more and more attention due to its characteristics of utilizing renewable resources and less environmental pollution. [0004...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12R1/22
Inventor 金平王领民王崇辉佟明友黎元生
Owner CHINA PETROLEUM & CHEM CORP
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