Method for producing glucosamine by fermentation

A technology for glucosamine and glucose, which is applied in the field of fermentation and production of glucosamine, which can solve problems such as low process efficiency, high production cost, and industrial pollution, and achieve the effects of increasing synthesis rate, increasing yield, and efficient fermentation production

Inactive Publication Date: 2020-01-03
SHANDONG RUNDE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production raw materials of acid hydrolysis and enzymatic hydrolysis come from the exoskeleton of fish, shrimp and crab, etc., and glucosamine is obtained by extracting chitin and chitosan, and then undergoing acid hydrolysis or enzymatic hydrolysis; but in the process of acid hydrolysis, a large amount of concentrated Hydrochloric acid will bring serious industrial pollution; the enzymatic hydrolysis method is to use chitosan to degrade the exoskeleton of fish, shrimp and crab. This process is inefficient and the production cost is high. Therefore, it is necessary to find a suitable microbial fermentation method to realize the industrialization of glucosamine Production is the current environment and market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Mix Escherichia coli, Saccharomyces cerevisiae, and Staphylococcus in a volume ratio of 3:0.8:1.2 and inoculate them in the seed medium for activation culture. Control the culture temperature at 37°C, shaker speed at 230 rpm, culture time for 12 hours, and adjust the pH value to 6.7 with ammonia water Inoculate the mixed starter into the fermentation medium containing glucose according to 10% inoculum size and carry out fermentation culture in the fermenter. The culture temperature is 37°C, the shaker speed is 230rpm, the culture time is 40h, and the pH value is adjusted to 6.7 with ammonia water. The glucosamine-containing fermentation liquid was obtained, and the glucose feeding rate was controlled to be 4L / h during the whole fermentation process, and the glucosamine content in the fermentation liquid was detected to be 80.2g / L.

Embodiment 2

[0022] Mix Escherichia coli, Saccharomyces cerevisiae, and Staphylococcus in a volume ratio of 3:0.5:1.5 and inoculate them in the seed medium for activation culture. The culture temperature is controlled at 36°C, the shaker speed is 250 rpm, the culture time is 12 hours, and the pH value is adjusted to 6.7 with ammonia water. Inoculate the mixed starter into the fermentation medium containing glucose according to 10% inoculation amount and carry out fermentation culture in the fermenter. The culture temperature is 35°C, the shaker speed is 250rpm, the culture time is 42h, and the pH value is adjusted to 6.7 with ammonia water. The glucosamine-containing fermentation liquid was obtained, and the glucose feed rate was controlled to be 3.5 L / h during the whole fermentation process, and the glucosamine content in the fermentation liquid was detected to be 76.8 g / L.

Embodiment 3

[0024] Mix Escherichia coli, Saccharomyces cerevisiae, and Staphylococcus in a volume ratio of 3:0.6:1.2 and inoculate them in the seed medium for activation culture. Control the culture temperature at 37°C, shaker speed at 240 rpm, culture time for 13 hours, and adjust the pH value to 6.9 with ammonia water Inoculate the mixed starter into the fermentation medium containing glucose according to 10% inoculum amount and carry out fermentation culture in the fermenter. The culture temperature is 35-38°C, the shaker speed is 240rpm, and the culture time is 35-48h, adjusted with ammonia water The pH value was 6.9, and the fermentation broth containing glucosamine was obtained. The glucose feed rate was controlled to be 4L / h during the whole fermentation process, and the glucosamine content in the fermentation broth was detected to be 78.1g / L.

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Abstract

The present invention discloses a method for producing glucosamine by fermentation. A mixed fermentation agent is prepared after escherichia coli, saccharomyces cerevisiae and staphylococcus are activated; a fermentation culture medium containing glucose is inoculated with the mixed fermentation agent, fermentation culture is conducted in a fermentation tank at a culture temperature of 35-38 DEG Cand culture time of 35-48 h to obtain fermentation liquid containing the glucosamine, and after separation and purification, the glucosamine product is obtained. The mixed fermentation agent realizeshigh-efficiency fermentation to produce the glucosamine, addition of cysteine can reduce production rate of glutamic acid during fermentation process and synthesis rate of the glucosamine is improved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for producing glucosamine by fermentation. Background technique [0002] Glucosamine (GleN) is an important hexosamine sugar, which is formed by replacing a hydroxyl group of glucose with an amino group. At present, there are two main types of glucosamine on the market, one is glucosamine hydrochloride, and the other is glucosamine sulfate. . Glucosamine Hydrochloride (D-Glucosamine Hydrochloride), molecular formula C 6 h 13 NO 5 ·HCl is a white crystal, odorless, slightly sweet, soluble in water, slightly soluble in methanol, insoluble in ethanol and other organic solvents, it has important physiological functions for the human body, participates in liver and kidney detoxification, exerts anti-inflammatory It has the function of protecting the liver from inflammation and has good curative effect on the treatment of rheumatoid arthritis an...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/26C12R1/19C12R1/865C12R1/44
CPCC12P19/26C12P39/00
Inventor 卢健行吴祥舟刘长峰马善丽卢建功卢建智
Owner SHANDONG RUNDE BIOTECH CO LTD
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