Miniaturized Staphylococcus aureus polypeptide of against drug-resistance and its uses and preparation method

Abstract

The invention discloses a midget anti drug-tolerance staphylococcus aureus polypeptide comprised of passage structure domain to form ion channel colicine and staphylococcus aureu pheromone, as well as opposite coded nucleotide sequence and recombination plasmid contained said sequence. Wherein, the plupeptide can not induce bacteria to generate trsditional drug-tolerance by the mechnism that it forms directly ion channel on cell film of target cell to kill bacteria. This invention has more single antimicrobial spectrum without effect to Gram-negative bacteria of escherichia coli, shows at least 500 times effect to pheneazonecillin, and can be used as synergist to beta-lactam antibiotics.

Description

technical field

[0001] The invention relates to a recombined miniaturized anti-drug-resistant Staphylococcus aureus polypeptide gene, a recombinant plasmid, a polypeptide and an application and preparation method thereof. Background technique

[0002] Bacterial infection is a major threat to human life and health. Since the advent of sulfonamides and penicillins, antibiotics have been invented by humans to achieve antibacterial purposes by inhibiting bacterial cell wall synthesis, inhibiting or interfering with bacterial nucleic acid and protein metabolism and synthesis pathways. However, these antibacterial pathways easily induce bacteria to mutate and produce antimicrobial resistance. Therefore, people have been devoting themselves to the development of new antibiotics.

[0003] The direct formation of ion channels on the bacterial cell membrane to cause bacterial death is one of the more promising directions for the development of antibiotics. According to this design c...

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