168 results about "Antibiogram" patented technology

The invention provides a high-efficiency compound microbial inoculum for fermenting beds, particularly relates to a compound microbial inoculum for the fermenting beds, and a preparation method and application thereof. The compound microbial inoculum is composed of five beneficial bacterium strains (Bacillus subtilis CGMCC1.769 and CGMCC1.210, lactobacillus plantarum CICC6073, Candida tropicalis CGMCC2.587 and Trichoderma koningii CGMCC3.4290) and metabolites thereof (amylase, protease, lipase, cellulase, organic acid, bacteriophage and the like). The compound microbial inoculum is prepared by using a submerged liquid mixed fermentation technique. The method comprises the following steps: carrying out liquid submerged fermentation on the lactobacillus plantarum at 35 DEG C for 12 hours, respectively inoculating Candida tropicalis and Trichoderma koningii, carrying out mixed fermentation at 30 DEG C for 12 hours, inoculating Bacillus subtilis, carrying out liquid submerged mixed fermentation for 24 hours, respectively controlling the fermented viable count at 1-2*10<9> cfu / mL, 5-8*10<8> cfu / mL, 3-5*10<8> cfu / mL, 3-5*10<9> cfu / mL and 2-5*10<9> cfu / mL, finishing fermentation, packaging and sealing to obtain the high-efficiency compound microbial inoculum for the fermenting beds. The invention also provides application of the high-efficiency compound microbial inoculum-a preparation method of padding material. The compound microbial inoculum has the advantages of high viable count, high biological activity and low impurity bacterium pollution; the compound microbial inoculum can obviously reduce the odor of pigsties and reduce the living possibility of mosquitoes and flies, thereby achieving the ecological culture goals of no pollution and zero discharge; and the process for manufacturing the ferment bed is simple, thereby saving the padding material, human power and the like.

The present invention relates to methods and systems for determining the antibiotic-resistance status of microorganisms. The invention further provides methods for determining the antibiotic-resistance status of microorganisms in situ within a single system.

Use of a macrolide antibiotic for the manufacture of a medicament for the treatment or prevention of a cancer selected from colorectal cancer, Desmoid tumor, bladder cancer, gastric cancer, and breast cancer, the macrolide antibiotic being one or more of : tylosin, pharmaceutically acceptable salts thereof, and derivatives thereof; erythromycin, pharmaceutically acceptable salts thereof, and derivatives thereof; oleandomycin, pharmaceutically acceptable salts thereof, and derivatives thereof; and spiramycin, pharmaceutically acceptable salts thereof, and derivatives thereof. Also provided are pharmaceutical compositions and methods for the treatment or prevention of the above mentioned cancers and methods for treating or preventing, in a mammal, a cancer that expresses a mutated APC gene.

The invention relates to Mulin acetate containing substituted squaric acid and an application thereof which belong to the technical field of medicines, specifically to substituted squaric acid containing Mulin acetate as shown in a general formula (I), pharmaceutically acceptable salt, a hydrate and an isomer thereof, wherein R1, R2, R3, R4, R5 and B are defined in the specification. The invention also relates to a preparation method of these compounds, a pharmaceutical composition containing these compounds and an application of these compounds in the preparation of drugs for treating or preventing bacteria and virus. As for staphylococcus aureus and Streptococcus equin MIC value, the compounds have an antibacterial effect 15-20 times higher than a control commercially-available antibiotic tiamulin in the test. And the compounds provided by the invention are effective antimicrobial agents. And the compounds provided by the invention are effective antimicrobial agents.

The invention belongs to the technical field of organic synthesis and pharmaceutical chemistry and especially relates to a preparation method of an animal antibiotic tulathromycin. According to the preparation method, azithromycin A and acetic anhydride which are used as raw materials are subjected to protection and oxidation to obtain oxide cyclic ketone; the intermediate is used for innovative addition of cyclic ketone and nitromethane; an addition product is reduced to obtain methyleneamine; direct condensation between methyleneamine and propionaldehyde is carried out; and reduction is conducted to obtain the high-purity target compound. Raw materials which are cheap and easily available are adopted. The preparation method has advantages of simple reaction, easily-controlled reaction process, high product purity, manageability, high yield, low cost and the like, and is suitable for large-scale industrial production.

The invention discloses a Pseudomonas aeruginosa vaccine and a preparation method thereof, which relate to a vaccine and a preparation method thereof. The invention solves the problems that the prior antibiotic is difficult to cure Pseudomonas aeruginosa infection, and a Pseudomonas aeruginosa vaccine with single valence can not satisfy the demand of clinical immunity. The vaccine consists of endotoxin of Pseudomonas aeruginosa P1, endotoxin of Pseudomonas aeruginosa P2, endotoxin of Pseudomonas aeruginosa P6 and toxoid of Pseudomonas aeruginosa P103 which are placed in physiological saline. The preparation method comprises the following steps: the endotoxins of Pseudomonas aeruginosaes are prepared; the toxoid of the Pseudomonas aeruginosa P103 is prepared; and the Pseudomonas aeruginosa vaccine can be obtained by mixing the endotoxins of the Pseudomonas aeruginosaes and the toxoid of the Pseudomonas aeruginosa P103. The Pseudomonas aeruginosa vaccine belongs to a polyvalent vaccine; the endotoxins of the Pseudomonas aeruginosa P1, the Pseudomonas aeruginosa P2 and the Pseudomonas aeruginosa P6 are adopted, so known type 11 Pseudomonas aeruginosa can be immunized; and the toxoid of the Pseudomonas aeruginosa P103 is added, so the immune effect of the vaccine to the Pseudomonas aeruginosaes is strengthened.

The invention discloses a synergist for improving the gram-positive-bacterium-resistant effect of nisin. Microdilution method drug susceptibility tests and chessboard type microdilution tests are used, tested bacteria comprise nine staphylococcus aureus food isolated strains, nine listeria monocytogenes food isolated strains and two quality-control strains for contrast, that is, staphylococcus aureus ATCC 29213 and listeria monocytogenes ATCC 19115, the effect caused by perilla oil on the antibacterial activity of the nisin is measured, and test results prove that the perilla oil has the effect of enhancing the gram-positive-bacterium-resistant activity of the nisin. The nisin is a polypeptide antibiotic substance extracted from a streptococcus lactis fermentation product, can be degraded by some proteases in human body digestive tracts easily, and has no adverse effect on colors, aromas, tastes and the like of food, and perilla is rich in alpha-linolenic acid and has effects of resisting oxidation, resisting ageing, resisting thrombus, preventing and treating cardiovascular diseases, resisting cancers, resisting allergies and resisting inflammations. High-nisin and the perilla oil are used as food preservatives, the additive amount of the high-nisin can be decreased, safe and reliable purposes can be achieved, and the health-care effect can be realized.

The invention discloses a biopesticide complex inoculant for root disease prevention and control of crops like cotton and a preparation method thereof. The biopesticide complex inoculant is microbial pesticide utilizing a powerful microbial combination and having efficient effect on root diseases of the crops like cotton. Specifically, microorganisms like white hook streptomyces generating gentamicin, streptomyces ambofaciens generating spiramycin and Congomycin, streptomyces cellulosae generating streptomycin and actinomycin, streptomyces hygroscopicus Jingang variant generating Jingangmycin and preventing and controlling rice sheath blight, streptomyces microaureus generating kasugamycin and preventing and controlling rice blast, streptomyces generating staphylomycin and anti-G+cell, streptomyces Jingyangensis generating long-acting stimulin and streptomyces rimosus generating tetraenes antibiotics are subjected to fermenting cultivation, and an improved culture medium formula is adopted to prepare 2 billion per ml biological unit. The biopesticide complex inoculant is used for seed soaking, seed mixing or being blended in biological seed fertilizer before seeding the crops like cotton to play a role in preventing and controlling root diseases of the crops like cotton, and can be used for root irrigation when cotton and other crops are infected by the diseases to prevent and control crop root diseases, and effective rate reaches 65-90%.