Immunomagnetic nano particle cell separator, and its preparing method and use
A magnetic nanoparticle, cell separator technology, applied in biochemical instruments, biochemical equipment and methods, bioreactor/fermenter combinations, etc., can solve the problem that there is no report on the structure of magnetic nanoparticle cell separators, and it is not convenient to separate CD34+ cells And other issues
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0030] The method for preparing immunomagnetic nanoparticle cell separator of the present invention comprises the following steps:
[0031] (1) Preparation of magnetic nanoparticles
[0032] Separately prepare FeSO with double distilled water 4 .7H 2 O and FeCl 3 .6H 2 O mixed solution and NaOH solution. In a mixed solution of iron salts, Fe 2+ The concentration of ions is 0.1-0.2mol / l, Fe 3+ The concentration of ions is 0.1-0.3 mol / l, and the concentration of NaOH solution is 2-3 mol / l. Under vigorous stirring, the NaOH solution whose volume is half of the volume of the mixed salt solution was slowly added dropwise into the mixed salt solution. Aging the obtained solid precipitate at 40°C-60°C for 12h, washing the precipitate several times with double distilled water, filtering and drying at 40°C-80°C for 24h, grinding in an agate mortar That is the product.
[0033] (2) Silica in γ-Fe 2 o 3 surface modification
[0034] Mix TritonX-100, n-hexanol and cyclohexane ...
Embodiment 1
[0049] Preparation method of inner core layer of magnetic nanoparticles
[0050] The core layer of magnetic particles was prepared by improved chemical co-precipitation, the specific method is as follows: prepare FeSO 4 .7H 2 O and FeCl 3 .6H 2 O mixed solution and NaOH solution. In a mixed solution of iron salts, Fe 2+ The concentration of ions is 0.1-0.2mol / l, Fe 3+ The concentration of ions is 0.1-0.3 mol / l, and the concentration of NaOH solution is 2-3 mol / l. Under vigorous stirring, the NaOH solution whose volume is half of the volume of the mixed salt solution was slowly added dropwise into the mixed salt solution. Aging the obtained solid precipitate at 40°C-60°C for 12h, washing the precipitate several times with double distilled water, filtering and drying at 40°C-80°C for 24h, and grinding it in an agate mortar Get the product.
Embodiment 2
[0052] Preparation method of core-shell nucleic acid magnetic nanoparticles
[0053] Mix TritonX-100, n-hexanol and cyclohexane uniformly at a ratio of 1:2:5 to form a transparent and stable microemulsion system. Put the above-mentioned microemulsion system in ultrasonic treatment for 30-60 minutes, then add 0.5g of γ-Fe to it 2 o 3 After 6 minutes of ultrasonic treatment, the supernatant was taken out and poured into a three-necked flask, and stirred for 30 minutes to make it uniform. Take 1ml of a certain concentration of concentrated ammonia water and dilute it with 2ml of double distilled water, slowly add it into the microemulsion that is constantly stirring after 30 minutes, and keep stirring for 30 minutes to make the ammonia water evenly dispersed in the microemulsion. After 1 hour, 1-3 ml of tetraethyl orthosilicate was added dropwise to the microemulsion, while stirring continuously for 10 hours, and the temperature of the system was kept between 15-30°C. Add ac...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More