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Simulated moving-bed chromatographic process for separating and purifying flavone from Chinese yam

A simulated moving bed, separation and purification technology, applied in the field of simulated moving bed chromatography to separate and purify flavonoids from Chinese yam, can solve problems such as no public reports, and achieve the effects of high yield, improved automation, and improved production environment

Active Publication Date: 2009-12-02
JIANGSU HANBON SCI & TECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no public report on the method of using simulated moving bed chromatography to separate and purify the flavonoids of Chinese yam

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] Example 1: Separation and purification of Chinese yam flavonoids on a simulated moving bed of Jiangsu Hanbang Technology Co., Ltd. according to the following steps:

[0014] a, get 10kg of 88% ethanol and ultrasonically extract 0.5kg to get the flavonoids in Chinese yam;

[0015] b. Add hexane according to the volume ratio of ethanol and hexane as 4:6, carry out liquid-liquid extraction at room temperature for 3 hours, filter, and evaporate the filtrate to dryness under reduced pressure to obtain the crude extract of Chinese yam flavonoids;

[0016] c. The crude extract of yam flavonoids is made into a feed solution and purified in a simulated moving bed; the crude extract of yam flavonoids is dissolved in methanol, and filtered through a 0.45 μm organic pump filter membrane to obtain a simulated moving bed feed solution with a concentration of 100 mg / ml; The eluent is a mixture of methanol and water, the volume ratio of methanol and water is 4:6;

[0017] d. Dissolve ...

example 2

[0021] Example 2: Separation and purification of Chinese yam flavonoids on a simulated moving bed of Jiangsu Hanbang Technology Co., Ltd. according to the following steps:

[0022] a. Get 17kg of 88% ethanol and ultrasonically extract the flavonoids in 0.8kg of Chinese yam;

[0023] b. Add hexane according to the volume ratio of ethanol and hexane as 4:6, carry out liquid-liquid extraction at room temperature for 4 hours, filter, and evaporate the filtrate to dryness under reduced pressure to obtain the crude extract of Chinese yam flavonoids;

[0024] c. The crude extract of yam flavonoids is made into a feed solution and purified in a simulated moving bed; the crude extract of yam flavonoids is dissolved in methanol, and filtered through a 0.45 μm organic pump membrane to obtain a simulated moving bed feed solution with a concentration of 200 mg / ml; The eluent is a mixture of methanol and water, the volume ratio of methanol and water is 4:6;

[0025] d. Dissolve the extract...

example 3

[0029] Example 3: Separation and purification of Chinese yam flavonoids on a simulated moving bed of Jiangsu Hanbang Technology Co., Ltd. according to the following steps:

[0030] a. Get 24kg of 88% ethanol and ultrasonically extract the flavonoids in 1.2kg of Chinese yam;

[0031] b. Add hexane according to the volume ratio of ethanol and hexane as 4:6, carry out liquid-liquid extraction at room temperature for 5 hours, filter, and evaporate the filtrate to dryness under reduced pressure to obtain the crude extract of Chinese yam flavonoids;

[0032] c. The crude extract of yam flavonoids is made into a feed solution and purified in a simulated moving bed; the crude extract of yam flavonoids is dissolved in methanol, and filtered through a 0.45 μm organic pump membrane to obtain a simulated moving bed feed solution with a concentration of 300 mg / ml; The eluent is a mixture of methanol and water, the volume ratio of methanol and water is 4:6;

[0033] d. Dissolve the extract...

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Abstract

The invention discloses a method for separating and purifying flavonoids from Chinese yam by simulated moving bed chromatography. First, 10-24 kg of 88% ethanol is used to ultrasonically extract the flavonoids from 0.5-1.2 kg of Chinese yam; secondly, the volume ratio of ethanol and hexane is 4: 6 Add hexane, perform liquid-liquid extraction at room temperature for 3-5 hours, and evaporate the filtrate to dryness under reduced pressure to obtain the crude extract of flavonoids from Chinese yam; The crude extract of yam flavonoids is filtered through a 045 μm organic filter membrane to obtain a sample solution with a concentration of 100-300 mg / ml; the eluent is a mixture of methanol and water with a volume ratio of 4:6; finally, the extraction of the simulated moving bed Dissolve the liquid in ethanol, dissolve in 30mL-40mL ethanol per gram of extract, add water and let stand for 20 minutes, then gradually cool down to minus 5-minus 12°C, crystallize for 6-9 hours, filter, wash the crystals with water, crystallize twice, and dry the precipitate in vacuum Obtain the pure product of yam flavonoids. The continuous production of the present invention greatly improves the automation level, work efficiency and production environment.

Description

technical field [0001] The invention relates to a method for separating and purifying natural medicines, in particular to a method for separating and purifying flavonoids from yam by simulated moving bed chromatography. Background technique [0002] Yam medicine is a perennial winding herbaceous vine of Dioscoreaceae. Modern medical research shows that yam medicine contains flavonoids, saponins, mucus, choline, starch, sugar, protein, tannin, various amino acids, vitamin C, etc. Its internal components are relatively complex. The current method of extracting yam flavonoids is to soak, filter, and extract to obtain crude products, but to meet the medicinal requirements, it must be further purified. Currently, the commonly used purification method is column chromatography with neutral alumina as a filler. Law. Column chromatography is a commonly used refining method in the pharmaceutical and food industries. The equipment is simple and low in cost. However, although this meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/28C07H17/07A61K36/8945
Inventor 张大兵孟强王艳王亚辉
Owner JIANGSU HANBON SCI & TECH CO
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