Freezing Ballantine smear inspection method

Abstract

A method for detecting Pasteur smear of frozen type includes using sampler to collect cell sample from neck of uterus and coating collected cell sample on clean glass plate, placing said glass plate in fixing liquid of aceto-alcohol and taking glass plate out after cell sample is fixed, soaking said glass plate on cell denaturation recovery liquid for 1-5 min., taking it out then placing it in freezer for refrigerating to make water content in cell sample be formed to be ice crystal, taking it out to carry out HE stain on it at normal temperature then making observation and diagnosis under microscope.

Description

technical field [0001] The invention relates to a frozen Pap smear detection method. Background technique [0002] At present, the traditional Pap smear technology has been used for nearly 50 years, and has made an extremely important contribution to the prevention and treatment of cervical cancer worldwide. The method it adopts is to use samplers such as cotton swabs and scrapers to collect cell samples from the cervix, directly smear them on glass slides, fix them in time, and then stain them directly. Although it is simple, quick and cheap. But it still has the following disadvantages: 1), cell collection is not comprehensive, smear cells overlap, after application of high-concentration ethanol or ethanol ethyl ether mixed fixative, cell shrinkage is obvious, mucus denaturation is solidified, and staining agent is difficult for penetrating mucus in the staining process Denatured membranes lead to poor staining of mucus-wrapped cells and poor nuclear plasma staining, esp...

AI Technical Summary

Problems solved by technology

But it still has the following disadvantages: 1), cell collection is not comprehensive, smear cells overlap, after application of high-concentration ethanol or ethanol ethyl ether mixed fixative, cell shrinkage is obvious, mucus denaturation is solidified, and staining agent is difficult for penetrating mucus in the staining process Denatured membranes lead to poor staining of mucus-wrapped cells and poor nuclear plasma staining, especially when the smear is thick and the cell resolution is poor in areas with significant cell overlap, which seriously affects observation and easily leads to missed diagnosis and misdiagnosis; 2) when fixed cells If the staining is not done in time, the cells are easily deformed, and the subsequent staining effect will be seriously affected

For this reason, although medical experts have successively invented a variety of cytological improvement techniques, which have a certain effect on solving some defects of traditional Pap smears, such as TCT, LCT, LPT and other techniques, but these techniques are complicated to operate and require special equipment and special tools. Consumables are expensive, difficult to popularize, and have great limitations in practical applications, especially when performing large-scale cell smears, the above-mentioned technical defects are more prominent