Preparation process of plant estrin-enterodiol

A technology of phytoestrogens and enteric diols, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficulty in obtaining enteric diols and low yields, etc.

Inactive Publication Date: 2007-07-18
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using linseed to biosynthesize enterodiol, not only the yield is very small, but also it is difficult to obtain pure enterodiol

Method used

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  • Preparation process of plant estrin-enterodiol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1), strain cultivation:

[0026] Add 1mg of 4,4'-dihydroxyenterodiol to 100mL of GAM broth medium, then add 0.1g of fresh feces from healthy people, and culture anaerobically at 35°C for 24 hours to obtain a bacterial culture solution; use this bacterial culture solution as a strain .

[0027] (2), prepare a kind of phytoestrogen-enterodiol:

[0028] "Add 30mg of 4,4'-dihydroxyenterodiol to 900mL of GAM broth medium, then add 100mL of the strain prepared according to the above step (1), and culture it anaerobically at 35°C for 1 day to obtain a culture solution. Use a concentration of 0.1 % (v / v) acetic acid water-saturated n-butanol extraction culture solution, the volume ratio of acetic acid water-saturated n-butanol solution to the strain culture solution is 1: 1. The extract is concentrated under reduced pressure, separated and purified by a silica gel column to obtain 20mg A phytoestrogen - enterodiol.

Embodiment 2

[0030] (1), strain cultivation:

[0031] Add 100mg of 4,4'-dihydroxyenterodiol to 600mL of GAM broth medium, then add 6.0g of fresh feces from healthy people, and incubate anaerobically at 37°C for 36 hours to obtain a bacterial culture solution; use this bacterial culture solution as a strain .

[0032] (2), prepare a kind of phytoestrogen-enterodiol:

[0033] Add 700 mg of 4,4'-dihydroxyenterodiol to 400 mL of GAM broth medium, then add 600 mL of the strain prepared according to the above step (1), and culture anaerobically at 37° C. for 5 days. The culture solution was extracted with 0.1% (v / v) acetic acid water-saturated n-butanol, and the volume ratio of the acetic acid water-saturated n-butanol solution to the culture solution was 1:1. The extract was concentrated under reduced pressure, separated and purified through a silica gel column to obtain 400 mg of a phytoestrogen-enterediol.

Embodiment 3

[0035] (1), strain cultivation:

[0036] Add 2000mg of 4,4'-dihydroxyenterodiol to 800mL of GAM broth medium, then add 160g of fresh feces from healthy people, and culture it anaerobically at 40°C for 48 hours to obtain a bacterial culture solution; this bacterial culture solution is used as the strain.

[0037] (2), prepare a kind of phytoestrogen-enterodiol:

[0038] Add 3000mg of 4,4'-dihydroxyenterodiol to 200mL of GAM broth medium, then add 800mL of the strain prepared in the above step (1), and culture anaerobically at 35-40°C for 10 days. The culture solution was extracted with 0.1% (v / v) acetic acid water-saturated n-butanol, and the volume ratio of the acetic acid water-saturated n-butanol solution to the culture solution was 1:1. The extract was concentrated under reduced pressure, separated and purified through a silica gel column to obtain 1500 mg of a phytoestrogen-enterediol.

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Abstract

The present invention relates to biotransformation process to synthesize intestinal diol as one kind of lignanoid plant estrogen. The biotransformation process synthesizes intestinal diol with human intestinal bacteria in 4, 4'-dihydroxy intestinal diol as substrate and under anaerobic condition. The process can produce intestinal diol in great amount and obtain pure (-)-intestinal diol. Intestinal diol has the bidirectional estrogen-like and estrogen-resisting regulation effect, and may be used in preventing and treating climacteric syndrome, osteoporosis, cardiac vascular diseases, cancer, etc.

Description

technical field [0001] The invention relates to a preparation method of phytoestrogen-enterodiol. Background technique [0002] At present, some endocrine blood diseases, such as osteoporosis, climacteric syndrome, senile dementia, etc., use estrogen replacement therapy to achieve better results. However, estrogen not only has side effects and contraindications, but also has the risk of inducing breast cancer and endometrial cancer in long-term use. Therefore, it has important practical significance and broad application prospects to find natural products from natural plants that have less toxic side effects and have estrogen-like effects to replace chemical synthetic products. [0003] There are two main types of phytoestrogens: isoflavones and lignans. Today, soybean isoflavones are the most thoroughly studied, and lignans were discovered relatively late. Among the known plants, linseed and burdock are high in content. In 1996, Rickard S et al. first discovered secoisol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12R1/01
Inventor 刘淑莹赵宇峰刘志强宋风瑞
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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