Method for preparing functional target disk of mass spectrum sample de-absorbed by ground substance assistant laser

A matrix-assisted laser and sample target technology, applied in the direction of selective adsorption, material separation, instruments, etc., can solve the problems of interference analysis and identification, loss of active sites, cumbersome target disk production process, etc., to achieve rich and uniform pores and easy derivatization , Rich and controllable effects on the outer surface

Inactive Publication Date: 2007-07-25
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

However, it should be pointed out that the above-mentioned process still exists: the production process of the corresponding matrix-assisted laser desorption mass spectrometer sample target disk is cumbersome, and Fe(III) is fixed and bound by the interaction of charges between ions, so it is difficult to avoid Fe(III) in the actual operation process. III) Problems such as the loss of active sites, directly interfere with the analysis and identification of target phosphorylated protein molecules

Method used

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  • Method for preparing functional target disk of mass spectrum sample de-absorbed by ground substance assistant laser

Examples

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Effect test

example 1-3

[0025] Example 1-3: During the synthesis process of the corresponding nano-zeolite molecular sieve, by adjusting the reactant FeCl 3 The proportion of the solution is added to obtain a series of different ratios of 0.1%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0%, and 4.5% in the skeleton structure. The heteroatom nano zeolite crystal material (crystal size is about 250-300nm) of iron metal element, according to the specific technical route described in the invention technical scheme (2)-(6), make the heteroatom nano zeolite crystal material synthesized , uniformly modified on the surface of the MALDI stainless steel sample target disc to prepare a new type of MALDI functional sample target disc whose surface is uniformly decorated with nano-zeolite molecular sieve crystal materials doped with different proportions of iron metal atoms in the skeleton structure. Subsequently, the new MALDI functional sample target plate was used to directly enrich and identify the presence ...

example 4

[0026] Example 4: In the synthesis process of the corresponding nano-zeolite molecular sieve, by adjusting the reactant GaCl 3According to the proportioning addition of the solution, the framework structure is fixedly doped with 0.1%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, 3.5%, and 4.0% of a series of heterogeneous gallium elements in different proportions. Atomic nano zeolite crystal material (crystal size is about 250-500nm), according to the specific technical route described in the technical solution of the invention (2)-(6), the framework structure is fixedly doped with different proportions of gallium atoms as active sites The dotted nano-zeolite crystal material was evenly modified on the surface of the MALDI stainless steel sample target plate to prepare the MALDI functional sample target plate, which directly enriched and identified the phosphorylated peptide with a low abundance and a mass number of 2061.062 in the enzymatic hydrolysis mixture of β casein. Relevant exp...

example 5

[0027] Example 5: By adjusting the composition ratio of heteroatom substances such as iron source, titanium source, and gallium source in the corresponding nano-zeolite molecular sieve synthesis reaction mixture, the iron-titanium-gallium-titanium double metal heteroatoms in the framework structure are simultaneously fixed and doped in different proportions as The nano zeolite crystal material of the active site, according to the specific technical route described in the technical scheme (2)-(6) of the invention, the nano zeolite molecular sieve material synthesized above is uniformly modified on the surface of the MALDI stainless steel sample target disk, and the MALDI functional The sample target plate directly enriches and simultaneously identifies the presence of low-abundance phosphorylated peptides with a mass number of 2061.062 in the β-casein hydrolysis mixture, and the existence of low-abundance phosphorylated peptides in the proteolysis mixture of other actual sample s...

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Abstract

A method for preparing mass spectrum sample function target plate of base material auxiliary laser deabsorption includes fixing nanozeolite molecular sieve doped with heteroatom in bodyframe structure, dipping mass spectrum stainless steel sample target plate of base material auxiliary laser deasorption in titanate coupler, placing dipped target plate in anhydrous ethyl acetate to let heteroatom nanozeolite crystal material suspension liquid be sufficiently dispersed then adding said liquid in sample-set hole of said sample target plate and baking said sample target plate.

Description

technical field [0001] The invention relates to a method in the technical field of biological detection, in particular to a method for preparing a matrix-assisted laser desorption mass spectrometry sample functional target plate for directly enriching and identifying low-abundance phosphorylated peptide / phosphorylated protein molecules. Background technique [0002] Protein phosphorylation is the most common and important post-translational modification of proteins in organisms. The reversible process of protein phosphorylation and dephosphorylation is involved in almost all life activities such as cell signal transduction, cell differentiation and cell growth in higher eukaryotes, and is associated with many major diseases that threaten human health, such as tumors, etc. closely related to development. The purpose of modern proteomics research is to study the expression of the entire gene at the level of translation and post-modification, so as to study the composition of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/08G01N30/06B01D15/08
Inventor 徐芳陆豪杰王德举杨芃原包慧敏
Owner SHANGHAI JIAO TONG UNIV
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