Method for preparing integral affinity chromatographic column from metal heteroatom type Nano zeolite

Abstract

A method for preparing nanozeolite complete affinity chromatographic column of metal heteroatom type includes forming iron source solution by iron source material, dropping organic template agent in titanium source material to from transparent solution being added with silicon source material to form turbid liquid, dropping iron source solution in turbid liquid being used as gel presoma reaction solution, heating said reaction solution to be dried gel reaction presoma being filled into empty chromatographic column and obtaining said affinity chromatographic column after washing and drying as well as baking.

Description

technical field [0001] The invention relates to a preparation method in the field of nanotechnology, in particular to a preparation method for a metal heteroatom type nano zeolite monolithic affinity chromatographic column. Background technique [0002] Immobilized metal ion affinity chromatography is a bioseparation technique that has been gradually developed and applied in the last two decades. Natural or synthetic polymer soft gel chromatography matrix materials such as dextran gel and agarose gel are commonly used in traditional fixed metal ion affinity chromatography separation techniques, which obviously have (1) poor mechanical strength and easy compression deformation , it is difficult to increase the elution speed; (2) there is a risk of breeding microorganisms; (3) for the soft gel affinity chromatography matrix, the flow rate is slow, and its filling in the chromatographic column is not uniform, which affects the large amount of target organisms in the process of ...

AI Technical Summary

Problems solved by technology

Natural or synthetic polymer soft gel chromatography matrix materials such as dextran gel and agarose gel are commonly used in traditional fixed metal ion affinity chromatography separation techniques, which obviously have (1) poor mechanical strength and easy compression deformation , it is difficult to increase the elution speed; (2) there is a risk of breeding microorganisms; (3) for the soft gel affinity chromatography matrix, the flow rate is slow, and its filling in the chromatographic column is not uniform, which affects the large amount of target organisms in the process of affinity chromatography separation. Disadvantages such as molecular diffusion process

At the same time, since the affinity ligands such as metal ions are fixed in the matrix of the corresponding affinity chromatography filler through chelation between ions, the "bottleneck" problems such as the loss of affinity ligands such as metal ions in the process will be eliminated. Direct interference affects the biochemical existence and stability of low-abundance key functional biomacromolecules in the target biological system, including phosphorylated peptides and phosphorylated protein molecules; In this mode, the process of pre-filling the affinity packing matrix into the corresponding affinity chromatography column is not only complicated and tedious, but also the filling process will directly affect the corresponding column length, column pressure, column efficiency and other factors, so that it can be further improved Large-scale practical application is limited

[0003] After searching for prior art documents, David.C.Hoth etc. published a paper titled "Metal oxidemonolithic column" ("Journal of ChromatographyA" ("Journal of Chromatography", 2005, volume 1079, page 392) in "Journal of ChromatographyA" ("Metal oxidemonolithic column" Metal Oxide Monolithic Chromatographic Column"), it is proposed to use specific chemical synthesis reaction process to directly form monolithic chromatographic separation column with inorganic materials as the matrix, but this monolithic chromatographic separation column cannot be directly applied to low-abundance phosphorylated peptides or Enrichment identification of phosphorylated protein molecules