Method for screening high egg-yield fowl
A high-yield egg and poultry technology, applied in chemical instruments and methods, immunoglobulin, animal/human protein, etc., can solve the problem of not being able to increase chicken production
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specific Embodiment
[0021] According to a specific embodiment of the present invention, the method includes collecting specimens from test poultry, and detecting the content of insulin-like growth factor I in the specimen with an antibody that specifically binds to the epitopic region of insulin-like growth factor I. Including at least amino acid residues 75 to 89 as shown in SEQ ID NO: 1, wherein the judgment of high-yielding poultry is based on whether the detected protein content in the specimen contains higher or lower amounts. Herein, a higher amount of protein is defined as an antibody that specifically binds to the antigenic determinant region of insulin-like growth factor I, including at least amino acid residues 75 to 89 as shown in SEQ ID NO: 1, selected in poultry flocks Detect poultry with high protein content, or eliminate poultry with low protein content. For example, when the farm owner wants to select 5% of the high-yielding layer birds in the flock as breeders, he will select 5% ...
example 1
[0035] Example 1 Searching for protein indicators of high-yielding chickens
[0036] In this experiment, 35-week-old Taiwanese red-feathered chickens were used. According to the total number of eggs laid by Taiwanese red-feathered chickens at 26 to 48 weeks, they were divided into high-production group and low-production group. As shown in Table 1, the high-production group Chickens in the group with an average egg production of approximately 117 eggs at 26 to 48 weeks were classified as a low egg production group with an average egg production of approximately 69 eggs at 26 to 48 weeks.
[0037] Table 1
[0038] group
Number of samples
Number of eggs (pcs)
High egg production group
19
117±1 *
low egg production group
18
69±4 *
[0039] * Significant degree of difference p<0.05
[0040] In order to find out the protein indicators related to egg production, blood samples were taken from each group of Taiwan red chick...
example 2
[0046] Example 2 Antibody Synthesis
[0047] The predicted antigenic determinant region of Example 1 is a 15-amino acid peptide derived from amino acid residues 75 to 89 shown in SEQ ID NO:1. This peptide has the sequence KPTGYGSSSRRLHHK located in the middle of insulin-like growth factor I with high hydrophilicity, flexibility and superficial region, and preferably has secondary protein structure, such as α-helix (α-helix) and β-plane (β-sheet) .
[0048] The peptide was synthesized in the form of multiple antigenic peptides. The molecular weight of this kind of peptide is about 8-18kDa. The central region of the multiple antigen peptide contains 7 lysine (lysine) bonds and 8 peptide chains extending outward. In the production of antibodies, it does not need to be combined with the carrying protein. independently as an immune antigen. All synthesized peptides must be analyzed by high-pressure liquid chromatography and mass spectrometry to confirm their quality. The pept...
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