Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparation of collagen / chitose supporting stand of composite hydroxylapatite

A technology of compounding hydroxyapatite and chitosan, which is applied in the fields of medical science and prostheses, can solve the problem of difficulty in obtaining cell active sites, and achieves good application prospects, convenient use and good biocompatibility.

Active Publication Date: 2007-12-05
无锡贝迪生物工程股份有限公司
View PDF0 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional bone repair materials are difficult to obtain cell active sites to promote the expression of normal activity of osteoblasts

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparation of collagen / chitose supporting stand of composite hydroxylapatite
  • Method for preparation of collagen / chitose supporting stand of composite hydroxylapatite
  • Method for preparation of collagen / chitose supporting stand of composite hydroxylapatite

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] 1) Collagen / chitosan is dissolved in the acetic acid solution that mass concentration is 3%, and the mass ratio of collagen and chitosan is 5: 5, and the collagen / chitosan solution that obtains mass concentration is 0.5%, injects into mold , freeze at -20°C for 2 hours, freeze at -20°C, freeze-dry in a lyophilizer for 24 hours, take it out from the mold, and then soak it in an acetic acid solution with a mass concentration of 0.3%. Fully washed with distilled water, placed in 0.25% aqueous glutaraldehyde solution for cross-linking at 4°C for 12 hours, and fully washed with triple distilled water to obtain a cross-linked collagen / chitosan scaffold. Figure 1 is a scanning electron micrograph of a cross-linked collagen / chitosan scaffold.

[0024] 2) Prepare 0.05mol / L CaCl 2 Aqueous solution and 0.03mol / L diammonium hydrogen phosphate solution; Step 1) gained cross-linked collagen / chitosan scaffold in 0.05mol / L CaCl 2 Take it out after soaking in the aqueous solution for ...

example 2

[0028] Step 1) is the same as step 1) of Example 1, but the mass ratio of collagen and chitosan is 9: 1;

[0029] Step 2) with the step 2 of example 1), prepared the collagen / chitosan support of depositing hydroxyapatite, but CaCl 2 The concentration of the aqueous solution is 0.1mol / L, and the concentration of the corresponding diammonium hydrogen phosphate solution is 0.06mol / L;

[0030] Step 3) is the same as step 3) of Example 1, but the mineralization time is 12 hours; FIG. 6 is a scanning electron micrograph of hydroxyapatite particles in the collagen / chitosan scaffold of composite hydroxyapatite after mineralization.

example 3

[0032] Step 1) is the same as step 1) of Example 1, but the mass ratio of collagen / chitosan is 8: 2;

[0033] Steps 2) to 3) are the same as steps 2) to 3) in Example 1; FIG. 7 shows the changes in water absorption of the sedimentation scaffold and the mineralization scaffold obtained under different calcium chloride solution infiltration conditions.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses preparation process of collagen / chitosan rack with compounded hydroxyapatite. The preparation process includes the steps of: preparing cross-linked collagen / chitosan rack, chemical depositing to compound hydroxyapatite onto the rack, and mineralizing to obtain active compound bone rack. The preparation process is simple and has abundant material source and high production efficiency. The collagen / chitosan rack in bone-like structure has introduced active hydroxyapatite, and possesses the advantages of high biocompatibility, excellent comprehensive performance, use convenience and other advantages. The collagen / chitosan rack with compounded hydroxyapatite can promote the migration, growth and differentiation of bone cell, is favorable to raising the efficiency of repairing damaged bone tissue, and possesses excellent application foreground.

Description

technical field [0001] The invention relates to a preparation method of a collagen / chitosan scaffold compounded with hydroxyapatite. Specifically, through the bionic simulation of human bone structure and function, collagen / chitosan and hydroxyapatite are used to simulate the extracellular matrix of bone cells, providing a method for preparing composite structural scaffolds for bone repair. Background technique [0002] Bone injury is a common disease nowadays. Many patients suffer from articular bone injuries caused by various bone and joint diseases such as rheumatism and rheumatoid or sports trauma. So far, clinically, there is still a lack of effective methods for repairing large-sized bone defects. Applying the methods and principles of regenerative medicine to repair bone tissue is an important means at present, and has achieved good results. Among them, bone repair scaffolds play a very important role in bone regeneration. [0003] Bone is a general term for a gro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44
Inventor 高长有马列赵海光沈家骢
Owner 无锡贝迪生物工程股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com