Bilirubin measuring freeze-drying calibration liquid and preparing method thereof
A technology of bilirubin and calibration solution, which is applied in the field of preparation of freeze-dried calibration solution for bilirubin determination, can solve the problems of turbidity, high residual moisture, easy to deteriorate quickly, etc., and achieve the effect of stable properties and good stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] Take 150ml of qualified healthy human serum, put the raw serum in a plastic bottle, put it in a refrigerator at -20°C for 12 hours, and then immediately put it at room temperature for 12 hours, and repeat the freezing and thawing process 7 times until most of the upper layer of the serum is clear. state. Use a pipette to carefully separate the upper layer without confusion; filter with dense filter paper, 0.45 μm filter membrane, and then 0.22 μm filter membrane to make it transparent and sterile lipoprotein-free serum. Take 96ml of the above serum (≈96g, the density of serum is close to the density of water) in a Erlenmeyer flask with a capacity of 200ml for later use.
[0026] Weigh 4mg of indirect reaction bilirubin, add 0.2mol / L Na 2 CO 3 Solution 1ml, mix well until no dry powder floats on the liquid surface, then add 1ml of methanol and shake well until completely dissolved and transparent; weigh 12mg of direct reaction bilirubin, add 2ml of water and mix until ...
Embodiment 2
[0037] Take 150ml of qualified healthy human serum, put the raw serum in a plastic bottle, put it in a refrigerator at -20°C for 12 hours, and then immediately put it at room temperature for 12 hours, and repeat the freezing and thawing process 7 times until most of the upper layer of the serum is clear. state. Use a straw to carefully separate the upper layer without confusion; filter with dense filter paper, 0.45 μm filter membrane, and 0.22 μm filter membrane respectively to make it a transparent sterile lipoprotein-free serum. Take 94ml (≈94g) of the above serum in a Erlenmeyer flask with a capacity of 200ml for later use.
[0038] Weigh 3mg of indirect reaction bilirubin, add 0.2mol / L Na 2 CO 3 Solution 1ml, mix well until no dry powder floats on the liquid surface, then add 1ml of methanol and shake well until completely dissolved and transparent; weigh 11mg of direct reaction bilirubin, add 4ml of water and mix until completely dissolved and transparent.
[0039] Add...
Embodiment 3
[0050] Take 150ml of qualified healthy human serum, put the raw serum in a plastic bottle, put it in a refrigerator at -20°C for 12 hours, and then immediately put it at room temperature for 12 hours, and repeat the freezing and thawing process 7 times until most of the upper layer of the serum is clear. state. Use a straw to carefully separate the upper layer without confusion; filter with dense filter paper, 0.45 μm filter membrane, and 0.22 μm filter membrane respectively to make it a transparent sterile lipoprotein-free serum. Take 96ml (≈96g) of the above serum in a Erlenmeyer flask with a capacity of 200ml for later use.
[0051] Weigh 5mg of indirect reaction bilirubin, add 0.2mol / L Na 2 CO 3 Solution 1ml, mix well until no dry powder floats on the liquid surface, then add 1ml of methanol and shake well until completely dissolved and transparent; weigh 13mg of direct reaction bilirubin, add 2ml of water and mix until completely dissolved and transparent.
[0052] Add...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More