Target automatic separation method based on immune magnetic beads and separating mechanism

Abstract

The present invention discloses an immuomagnetic bead-based target automatic separation method and a separation device, the separation device consists of a mixing unit, a separation unit, and a control unit. A sample waiting to be detected and the immuomagnetic bead are charged into a sample pipe, and under different combinations of uniform speed rotation, the variable speed rotation, the static enrichment or the forward and reverse rotation states of the sample pipe, the target in the sample waiting to be detected and the antibody fixed on the immuomagnetic bead generate an idiosyncratic reaction; under a fixed magnetic field, the immuomagnetic bead and the target in the detected sample captured by the immuomagnetic bead are adsorbed to the inner wall of the sample pipe; the immuomagnetic bead and the target in the detected sample captured by the immuomagnetic bead are collected for biological detection or tray loading. Through the idiosyncratic reaction generated between the detected target in the sample pipe and the antibody fixed on the immuomagnetic bead, the present invention can accurately separate and screen the target detected object, and achieve the optimum mixing effect through the combination of different mixing patterns, thereby the present invention is widely applied to the preprocess of samples.

Description

technical field [0001] The invention relates to an automatic target separation method and separation device based on immunomagnetic beads. Background technique [0002] As we all know, sample pretreatment is a very important means, and its success is crucial to the detection of samples. The method of using immunomagnetic particles (magnetic beads) to screen and separate targets such as microorganisms, cells, and chemical elements in food, clinic, soil, and environment has been more and more affirmed. Due to the rapidity, high concentration ratio, and simple equipment requirements of the immunomagnetic bead separation / screening method, it is widely used in practice. Currently, immunomagnetic separation / screening methods generally consist of two steps: mixing and separation. The general mixer can only rotate in one direction, and the rotation speed is manually adjusted, and the mixing time can only be controlled by humans; the separation equipment is generally composed of pe...

AI Technical Summary

Problems solved by technology

The general mixer can only rotate in one direction, and the rotation speed is manually adjusted, and the mixing time can only be controlled by humans; the separation equipment is generally composed of permanent magnets and brackets, and the separation time is also controlled manually

Since these two key steps are operated separately, the separation effect is affected by many aspects, and it is necessary to purchase multiple devices to complete a separation operation. These factors greatly affect the pretreatment effect

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