Target automatic separation method based on immune magnetic beads and separating mechanism

An immune magnetic bead and automatic separation technology, which is applied in magnetic separation, solid separation, chemical instruments and methods, etc., can solve the problems of treatment effect, separation effect, mixing time can only be controlled by humans, etc.

Inactive Publication Date: 2008-02-06
ZHEJIANG UNIV
View PDF0 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The general mixer can only rotate in one direction, and the rotation speed is manually adjusted, and the mixing time can only be controlled by humans; the separation equipment is generally composed of permanent magnets and brackets, and the separation time is a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Target automatic separation method based on immune magnetic beads and separating mechanism
  • Target automatic separation method based on immune magnetic beads and separating mechanism
  • Target automatic separation method based on immune magnetic beads and separating mechanism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The present invention will be further described below in conjunction with drawings and embodiments.

[0019] As shown in Fig. 1, Fig. 2, Fig. 3, Fig. 4, the present invention comprises mixing unit 1, separation unit 2 and control unit 3; Wherein:

[0020] 1) Mixing unit 1, 4 sample tubes 101, 102, 103, 104 are respectively fixed in the respective sample holes 105, 106, 107, 108 on the mixing sample plate 111, and one end of the mixing sample plate 111 passes through the mixing motor 109 The first coupling 112 is connected, and the other end of the mixing sample plate 111 is connected to the casing through the bearing 110; the mixing motor 109 is electrically connected to the control unit 3 through the control cable 303;

[0021] 2) Separation unit 2, 4 separation magnets are respectively fixed on the separation transmission plate 205, and the 4 separation magnets correspond to the 4 sample tubes fixed on the mixing sample plate 111 one by one, and one end of the separat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses an immuomagnetic bead-based target automatic separation method and a separation device, the separation device consists of a mixing unit, a separation unit, and a control unit. A sample waiting to be detected and the immuomagnetic bead are charged into a sample pipe, and under different combinations of uniform speed rotation, the variable speed rotation, the static enrichment or the forward and reverse rotation states of the sample pipe, the target in the sample waiting to be detected and the antibody fixed on the immuomagnetic bead generate an idiosyncratic reaction; under a fixed magnetic field, the immuomagnetic bead and the target in the detected sample captured by the immuomagnetic bead are adsorbed to the inner wall of the sample pipe; the immuomagnetic bead and the target in the detected sample captured by the immuomagnetic bead are collected for biological detection or tray loading. Through the idiosyncratic reaction generated between the detected target in the sample pipe and the antibody fixed on the immuomagnetic bead, the present invention can accurately separate and screen the target detected object, and achieve the optimum mixing effect through the combination of different mixing patterns, thereby the present invention is widely applied to the preprocess of samples.

Description

technical field [0001] The invention relates to an automatic target separation method and separation device based on immunomagnetic beads. Background technique [0002] As we all know, sample pretreatment is a very important means, and its success is crucial to the detection of samples. The method of using immunomagnetic particles (magnetic beads) to screen and separate targets such as microorganisms, cells, and chemical elements in food, clinic, soil, and environment has been more and more affirmed. Due to the rapidity, high concentration ratio, and simple equipment requirements of the immunomagnetic bead separation / screening method, it is widely used in practice. Currently, immunomagnetic separation / screening methods generally consist of two steps: mixing and separation. The general mixer can only rotate in one direction, and the rotation speed is manually adjusted, and the mixing time can only be controlled by humans; the separation equipment is generally composed of pe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/34G01N1/28B03C1/32
Inventor 叶尊忠应义斌王剑平李延斌饶秀勤蒋焕煜蒋雪松
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap