Quantitative determination method for fluorescent whitening agent VBL, ABP for tobacco

A fluorescent whitening agent, quantitative determination technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unable to eliminate interference, unable to determine the type of fluorescent whitening agent, etc.

Active Publication Date: 2008-02-13
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method can only measure the total amount of fluorescent whitening agent, but cannot determine the type of fluorescent whitening a...

Method used

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  • Quantitative determination method for fluorescent whitening agent VBL, ABP for tobacco
  • Quantitative determination method for fluorescent whitening agent VBL, ABP for tobacco
  • Quantitative determination method for fluorescent whitening agent VBL, ABP for tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Column: Alltima C 18 Column, 150 mm x 4.6 mm, 5 μm.

[0067] Mobile phase: methanol / water: 45 / 55 (V / V), before HPLC analysis, filter through a 0.45 μm filter membrane and vacuum degassing.

[0068] Mobile phase flow rate: 1.0 mL / min.

[0069] Detection wavelength: excitation wavelength 350nm, emission wavelength 435nm.

[0070] Extraction solution: N,N-dimethylformamide.

[0071] Extraction time: 30min.

[0072] Extraction temperature: 40°C.

[0073] Use stainless steel scissors to cut the sample of tipping paper for cigarettes to 1mm×1mm, weigh 0.5g of the paper sample into a 50mL stoppered test tube, add 25.0mL of N,N-dimethylformamide to completely infiltrate the paper sample, and place 1 hour, ultrasonic oscillation at 40°C for 30 minutes. The oscillating solution was filtered through a 0.45 μn membrane filter, and the filtrate was used for later use. The above operations should be carried out in the dark as much as possible.

[0074] Use a microsampler or a...

Embodiment 2

[0080] Column: Alltima C 18 Column, 150 mm x 4.6 mm, 5 μm.

[0081] Mobile phase: methanol / water: 45 / 55 (V / V), before HPLC analysis, filter through a 0.45 μm filter membrane and vacuum degassing.

[0082] Mobile phase flow rate: 1.0 mL / min.

[0083] Detection wavelength: excitation wavelength 350nm, emission wavelength 435nm.

[0084] Extraction solution: N,N-dimethylformamide.

[0085] Extraction time: 30min.

[0086] Extraction temperature: 40°C.

[0087] Use stainless steel scissors to cut the filter rod forming paper sample to 1mm×1mm, weigh 0.5g of the paper sample into a 50mL stoppered test tube, add 25.0mL of N,N-dimethylformamide, soak overnight, and make the paper sample fully Infiltration, ultrasonic vibration at 40°C for 30min. The oscillating liquid was filtered through a 0.45 μm filter membrane, and the filtrate was used for later use.

[0088] Use a microsampler or autosampler to measure 10 μL of the sample treatment solution and inject it into the high pe...

Embodiment 3

[0093] Column: Alltima C 18 Column, 150 mm x 4.6 mm, 5 μm.

[0094] Mobile phase: methanol / water: 45 / 55 (V / V), before HPLC analysis, filter through a 0.45 μm filter membrane and vacuum degassing.

[0095] Mobile phase flow rate: 1.0 mL / min.

[0096] Detection wavelength: excitation wavelength 350nm, emission wavelength 435nm.

[0097] Extraction solution: N,N-dimethylformamide.

[0098] Extraction time: 30min.

[0099] Extraction temperature: 40°C.

[0100] Use stainless steel scissors to cut the samples of cigarette paper and filter rod forming paper to 1mm×1mm respectively, weigh 0.5g of the paper sample into a 50mL stoppered test tube, add 25.0mL of N,N-di-methylformamide, soak overnight , to fully infiltrate the paper sample, and vibrate ultrasonically at 40°C for 30min. The oscillating liquid was filtered through a 0.45 μm filter membrane, and the filtrate was used for later use.

[0101] Use a microsampler or autosampler to measure 10 μL of the sample treatment so...

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Abstract

A quantitative determination method of fluorescent whitening agents VBL and ABP used in cigarette paper is characterized in that the method mainly includes steps mention below. The first step is to determine the chromatographic conditions. A chromatographic column is C18, 5Mu m column of 150mm*46mm. A mobile phase is methane or water with 45/55(V/V). The flow speed of the mobile phase is 1.0mL/min. The detection wavelengths include an excitation wavelength of 350mm and an emission wavelength of 425mm. The second step is the detection step, which is also a preparation step of a calibration curve. Treatment liquid for paper sample to be detected is injected in a high performance liquid chromatograph. The quality is determined according to the reserved time and the quantity is determined according to the peak area. The invention provides a quantitative determination method of fluorescent whitening agents VBL and ABP used in cigarette paper, which has the advantages of accurate detection and simple operation. The qualitative and quantitative detection of the fluorescent whitening agents VBL and ABP in cigarette paper, filter rod forming paper and cigarette tipping paper is made to be convenient for industry technicians. And potential damages on physical health of customers caused by excess whitening agent can be prevented.

Description

technical field [0001] The invention relates to a method for quantitative determination of fluorescent whitening agents VBL and ABP in cigarette paper, in particular to the determination of fluorescent whitening agents VBL and fluorescent whitening agents in cigarette paper, filter rod forming paper and tipping paper for cigarettes by using high performance liquid chromatography. Whitening agent ABP content. Background technique [0002] Cigarette paper, filter rod forming paper, and tipping paper for cigarettes are essential raw materials for the production of cigarettes. In order to pursue high profits, individual manufacturers use inferior, unhygienic or even banned solvents, pigments, and fluorescent enhancers. Raw materials such as whitening agent, methanol, and xylene are used to produce cigarette paper, filter rod forming paper, and tipping paper for cigarettes, causing potential harm to consumers’ health (Shen Yongjia, Li Hongbin, Lu Wei, etc. Fluorescent whitening a...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02
Inventor 谢剑平姚效元韩云辉范黎王燕李栋李青常朱珂
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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