Culture medium for inducing clumping bud of sweet potato

A culture medium and bud induction technology, applied in plant regeneration, plant cells, horticulture, etc., can solve the problem of low reproduction coefficient, achieve the effect of increasing the reproduction coefficient and improving the efficiency of tissue culture

Inactive Publication Date: 2008-03-12
JIANGSU XUHUAI DISTRICT XUZHOU AGRI SCI INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the problem of low reproductive coefficient in the tissue culture of sweet potato shoot tips,

Method used

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  • Culture medium for inducing clumping bud of sweet potato

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1

[0018] Medium formula:

[0019] MS 1L

[0020] KT 1.0mg

[0021] 6-BA 2.0mg

[0022] IAA 2.0mg

[0023] CH 200mg

[0024] sucrose 20g

[0025] Agar 6g.

Example Embodiment

[0026] Example 2:

[0027] MS 1L

[0028] KT 2.0mg

[0029] 6-BA 2.0mg

[0030] IAA 2.0mg

[0031] CH 200mg

[0032] sucrose 20g

[0033] Agar 6g.

Example Embodiment

[0034] Example 3:

[0035] MS 1L

[0036] KT 1.0mg

[0037] 6-BA 1.0mg

[0038] IAA 0.2mg

[0039] CH 200mg

[0040] sucrose 20g

[0041] Agar 6g.

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PUM

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Abstract

The present invention relates to a culture medium for inducing sweet potato clustered shoots belonging to the sweet potato tissue culture technology. The culture medium is formed by dissolving the kinetin KT, 6-benzyl purine 6-BA, heteroauxing IAA, hydrolyzed casein CH, cane sugar and agar into the basic culture medium MS; the quantity of every part is that MS 11, 1.0-2.0 mg of KT, 1.0-2.0 mg of 6-BA, 0.2-2.0mg of IAA, CH 200mg, 20g of cane sugar and 6g of agar. The active effects of the present invention are that a stem tip meristematic tissue is utilized to induce 8.6 shoots and the inducement rate is 860 percent, which obviously promotes the tissue culture efficiency.

Description

technical field [0001] The invention relates to a medium for inducing clustered buds of sweet potato, which belongs to the technology of sweet potato tissue culture. In particular, a medium for inducing clustered shoots from the shoot apical meristem of sweet potato. Background technique [0002] The common method of culturing and propagating sweet potato shoot apical meristem is to induce differentiation of one shoot apical meristem into one bud, which is labor-intensive, time-consuming and inefficient. In the tissue culture and rapid propagation of many plants, the technology of inducing clustered buds is used to increase the reproduction coefficient and achieve twice the result with half the effort. Contents of the invention [0003] The purpose of the present invention is to overcome the problem of low reproduction coefficient in the tissue culture of sweet potato shoot tips, and provide a medium for efficiently inducing clustered buds of sweet potato, which is used f...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 张允刚唐君赵冬兰
Owner JIANGSU XUHUAI DISTRICT XUZHOU AGRI SCI INST
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