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Method for establishing rapid propagation system of tripterygium hypoglaucum

A technology of Begonia and system, applied in the field of rapid propagation system of Begonia in Kunming, which can solve the problems of natural regeneration difficulties, achieve the effects of shortening the cultivation time and cost, increasing the speed of rapid propagation, and avoiding the variation of tissue culture seedlings

Active Publication Date: 2015-03-25
嘉兴芸诗娇电子商务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Natural renewal is very difficult

Method used

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  • Method for establishing rapid propagation system of tripterygium hypoglaucum

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Experimental program
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Embodiment 1

[0022] Step 1. First, clean the terminal buds or axillary buds on the Kunming Begonia stems under running water, then soak them in 80% ethanol solution for 1 minute, sterilize with mercury for 8 minutes, and use sterile water to clean them repeatedly, then cut off the Kunming Begonias The terminal bud on the stem or the edge part of the axillary bud obtains the terminal bud or the axillary bud on the Kunming Mountain crabapple stem that has been processed; Under the conditions of 28°C, light intensity of 2600lux, and light time of 15h / d, small buds of Begonia Kunming can be obtained by culturing for 10 days; wherein, the induction medium is: with N 68 As the basic medium, add 0-1mg / L isopentenyl adenine, 1.0mg / L naphthaleneacetic acid, 2.0g / L gibberellin, 1.0mg / L thiadidiazuron, 20g / L sucrose and 4.0g / L Agar, adjust the pH value of the induction medium to 5.9;

[0023] Step 2: Put Kunming mountain crabapple buds in the subculture medium, and cultivate them for 28 days under t...

Embodiment 2

[0027] Step 1. First, clean the terminal buds or axillary buds on the stems of Kunming Begonia under running water, then soak them in 85% ethanol solution for 1.5 minutes, sterilize with mercury for 10 minutes, use sterile water to clean them repeatedly, and then cut off the Kunming Begonias The terminal bud on the stem or the edge part of the axillary bud obtains the terminal bud or the axillary bud on the Kunming Mountain crabapple stem that has been processed; Under the conditions of 30°C, light intensity of 2800lux, and light time of 14h / d, Kunming mountain crabapple buds can be obtained by culturing for 12 days; wherein, the induction medium is: with N 68 As basic medium, add 1.5mg / L isopentenyl adenine, 1.0mg / L naphthaleneacetic acid, 2.5g / L gibberellin, 2.0mg / L thiadidiazuron, 35g / L sucrose and 5.0g / L agar , adjust the pH value of the induction medium to 6.0;

[0028] Step 2: Place Kunming mountain crabapple buds in the subculture medium, and cultivate them for 30 days...

Embodiment 3

[0032] Step 1. First, clean the terminal buds or axillary buds on the stems of Kunming Begonia under running water, then soak them in 85% ethanol solution for 1 minute, sterilize them with mercury for 8 minutes, and use sterile water to clean them repeatedly, then cut off the Kunming Begonias The terminal bud on the stem or the edge part of the axillary bud obtains the terminal bud or the axillary bud on the Kunming Mountain crabapple stem that has been processed; Under the conditions of 32°C, light intensity of 3000lux, and light time of 13h / d, Kunming mountain crabapple buds can be obtained by culturing for 12 days; wherein, the induction medium is: with N 68 As the basic medium, add 0.5mg / L isopentenyl adenine, 0.5mg / L naphthaleneacetic acid, 3.0g / L gibberellin, 0.5mg / L thiadizuron, 50g / L sucrose and 6.0g / L agar , adjust the pH value of the induction medium to 6.2;

[0033] Step 2: Put the small buds of Kunming Begonia in the subculture medium, and cultivate them for 25 da...

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Abstract

The invention discloses a method for establishing a rapid propagation system of tripterygium hypoglaucum. The method comprises the following steps: seeding terminal buds or axillary buds of tripterygium hypoglaucum into an induction culture medium to induce sprouting, subsequently transferring and transplanting small buds of tripterygium hypoglaucum into a subculture medium and a rooting medium in sequence to culture continuously, thereby obtaining root tissue culture seedlings of tripterygium hypoglaucum, and further transplanting the root tissue culture seedlings into a seedbed to harden the seedlings. The tissue culture method disclosed by the invention is simple and convenient, simple in step and convenient to operate, the prepared tripterygium hypoglaucum seedlings are healthy and strong, high in growing ability and applicable to large-scale production, the culture time is effectively shortened and the production cost is also lowered.

Description

technical field [0001] The invention relates to a method for rapid propagation of plants, in particular to a method for establishing a rapid propagation system of Kunming mountain crabapple. Background technique [0002] Kunming mountain crabapple (Tripterygium hypoglaucum (Lv.1) Hutch) is a deciduous woody vine of the genus Tripterygium in the family Celastraceae. In "Southern Yunnan Materia Medica", "Zijinpi" is its drug name; Kunming mountain crabapple is commonly known as "torch flower", which was first recorded in Li Shizhen's "Compendium of Materia Medica" in the section of poisonous weeds in the grass. Torch Flower". Different ethnic groups use the medicinal parts of the plant as roots, root bark and stems. Folks often use woody canes as medicines, or use the roots and whole plants as regular supplies of Vajra vine; root bark, young branches and leaves are often used by farmers in the countryside. Used as botanical insecticide or pesticide. Kunming Begonia is used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 霍丽妮陈睿李培源苏炜苏钛王杰鹏
Owner 嘉兴芸诗娇电子商务有限公司
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