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A kind of microtuber propagation method for cultivating immature embryos of Polygonatum polyflora

A technology for culturing Polygonatum polyflora and immature embryos, which is applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions to achieve the effects of resistance to transportation, fast cultivation and strong differentiation ability

Active Publication Date: 2020-04-24
NINGDE NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a microtuber propagation method for cultivating Polygonatum polyflora immature embryos, which can quickly breed Polygonatum polyflora seedlings in an industrialized and large-scale manner, and solves the problem that a large number of provenances need to be rapidly propagated in the artificial cultivation of Polygonatum polyflora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The microtuber propagation method of Polygonatum multiflora immature embryo culture comprises the following steps:

[0017] (1) Selection of excellent individual plants of Polygonatum multiflora: Select a single plant of Polygonatum multiflora with strong growth, strong disease resistance, large rhizomes, good quality and excellent agronomic characteristics, observe its flowering and fruiting, and test the fruit 1 month before the fruit matures. , Harvest the immature fruit of a single plant.

[0018] (2) Extraction of immature seeds and immature embryos: The immature fruit of a single plant of Polygonatum multiflora is disinfected according to the routine tissue culture explant disinfection procedure. In a sterile environment, the fruit is opened, the seeds are taken out, and the seeds are peeled off. Take the young and complete embryos from the seeds.

[0019] (3) Induction culture of immature embryos: insert aseptically stripped young embryos into induction growth m...

Embodiment 2

[0025] The microtuber propagation method of Polygonatum multiflora immature embryo culture comprises the following steps:

[0026] (1) Selection of excellent individual plants of Polygonatum polyflora: Select a single plant of Polygonatum polyflora with robust growth, strong disease resistance, large rhizomes, good quality and excellent agronomic characteristics, observe its flowering and fruiting, and 2 months before the fruit matures , Harvest the immature fruit of a single plant.

[0027] (2) Extraction of immature seeds and immature embryos: The immature fruit of a single plant of Polygonatum multiflora is disinfected according to the routine tissue culture explant disinfection procedure. In a sterile environment, the fruit is opened, the seeds are taken out, and the seeds are peeled off. Take the young and complete embryos from the seeds.

[0028] (3) Induction culture of immature embryos: insert the aseptically stripped young embryos into induction growth medium MS+2.0m...

Embodiment 3

[0034] The microtuber propagation method of Polygonatum multiflora immature embryo culture comprises the following steps:

[0035] (1) Selection of excellent individual plants of Polygonatum multiflora: Select a single plant of Polygonatum multiflora with strong growth, strong disease resistance, large rhizomes, good quality and excellent agronomic characteristics, observe its flowering and fruiting, and test the fruit 1 month before the fruit matures. , Harvest the immature fruit of a single plant.

[0036] (2) Extraction of immature seeds and immature embryos: The immature fruit of a single plant of Polygonatum multiflora is disinfected according to the routine tissue culture explant disinfection procedure. In a sterile environment, the fruit is opened, the seeds are taken out, and the seeds are peeled off. Take the young and complete embryos from the seeds.

[0037] (3) Induction culture of immature embryos: insert the aseptically stripped young embryos into induction grow...

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Abstract

The invention provides a polygonatum cyrtonema immature embryo culture micro-stem tuber breeding method. The method comprises the following steps: selection of excellent single plant of polygonatum cyrtonema, stripping of immature seed immature embryos, induction culture of immature embryos, induction formation of micro stem tuber, propagation of micro stem tuber, and direct transplanting of micro stem tuber for seedling establishment. The polygonatum cyrtonema immature embryo culture has the advantages of rapid growth, strong differentiation force, and large propagation coefficient, and propagation and transplanting of the micro stem tuber have the advantages of fast propagation speed, large quantity, short period, convenient transplanting, storage resistance, transport resistance, direct transplanting, simple management and high survival rate; so that the seedling growing problems of slow breeding and seeding formation of the polygonatum cyrtonema seeds, low rhizome propagation coefficient and high cost in the prior art can be solved, polygonatum cyrtonema seedling can be rapidly breed, and the method is suitable for industrial large-scale production.

Description

technical field [0001] The invention belongs to the field of medicinal plant breeding, and in particular relates to a microtuber propagation method for culturing immature embryos of Polygonatum polyflora. [0002] technical background [0003] Polygonatum multiflora is a dual-purpose Chinese herbal medicine for medicine and food. It has the functions of nourishing qi and nourishing yin, invigorating the spleen, nourishing the lungs, and benefiting the kidney. Polygonatum has become the main body of market supply. Polygonatum can be propagated mainly by seeds and rhizomes. Because of the long dormant period after the seeds are mature, the seeds germinate and grow slowly, the natural germination rate is low, the time for seedlings and seedlings is long, it is difficult to manage, and the seeds germinate Only grow into a single small plant, harvest a single small rhizome, the propagation speed is slow, time-consuming, and less used in production; artificial cultivation mostly u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 叶祖云陶心想许雅彬吴文标郭团玉
Owner NINGDE NORMAL UNIV
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