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Duganella bacterium and uses thereof

A technology of Duganella and strains, applied in the direction of bacteria, fermentation, etc., can solve the problems of limited species and few applied research, and achieve the effect of fast growth rate, good application prospect and good biological activity.

Inactive Publication Date: 2010-06-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the number of known species of Duanella is limited, and its application is rarely studied

Method used

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  • Duganella bacterium and uses thereof
  • Duganella bacterium and uses thereof
  • Duganella bacterium and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Isolation and identification of Duganella sp. B2 CGMCC № 2056

[0029] 1. Sample collection

[0030] Collect soil samples from Xinjiang Glacier No. 1

[0031] 2. Isolation and screening of strains

[0032] Prepare separation plate medium: 5g peptone, 3g beef extract, 20g agar, 1000ml water, pH 7.0. The above medium is 1.05kg / cm 2 , 121℃, autoclave for 20 minutes.

[0033] Make the soil sample into a 2% (2g / 100ml) suspension, and dilute it to 2×10 in 10-fold increments 8 , Take 0.2mL of each dilution sample solution and evenly spread on the above separation medium plate, culture for 7 days at 25°C, observe that there is a single blue colony, select the colony, conduct streaking separation, and pick again after culture Take a single colony of bacteria for streaking separation, repeat the above steps until it is a pure strain (detected with a microscope, the cell shape is consistent as a pure strain), and this strain is named B2, which is Duganella sp. B2 CGMCC №2056. ...

Embodiment 2

[0047] Example 2. Using Duganella sp. B2 CGMCC № 2056 to produce purple bacterin

[0048] 1. Strain culture

[0049] Prepare plate medium: 5g peptone, 3g beef extract, 20g agar, add water to 1000ml, adjust pH 7.0. Sterilize the plate medium at 121°C for 15 minutes, pour the plate, and insert Duganella sp. B2 CGMCC No. 2056 after cooling, and incubate at 25°C for 72 hours.

[0050] Prepare seed liquid medium (content per liter): 5g peptone, 3g beef extract, and adjust pH to 7.0. A single Duganella sp. B2 CGMCC № 2056 colony was picked from the plate and inoculated in a sterile liquid medium, and cultured at a temperature of 25° C., at a rotation speed of 200 rpm (rotation radius of 32 mm). After 24 hours of culture, the bacteria are in the logarithmic growth phase.

[0051] Prepare fermentation liquid culture medium: starch 15g, ferrous sulfate 0.03g, potassium nitrate 1g, dipotassium hydrogen phosphate 0.7g, magnesium sulfate 0.5g, tryptophan 0.5g and water to 1000ml, pH is 7.0. Pu...

Embodiment 3

[0086] Example 3. Production of violacein by Duganella sp. B2 CGMCC № 2056

[0087] 1. Cultivation of strains

[0088] Prepare plate medium: 5g peptone, 3g beef extract, 20g agar, add water to 1000ml, adjust pH 7.0. Sterilize the plate medium at 121°C for 15 minutes, pour the plate, and insert Duganella sp. B2 CGMCC No. 2056 after cooling, and incubate at 25°C for 72 hours.

[0089] Prepare seed liquid medium (content per liter): 5g peptone, 3g beef extract, and adjust pH to 7.0. A single Duganella sp. B2 CGMCC № 2056 colony was picked from the plate and inoculated in a sterile liquid medium, and cultured at a temperature of 25° C. with a stirring speed of 200 rpm (rotation radius of 32 mm). After 24 hours of culture, the bacteria are in the logarithmic growth phase.

[0090] Prepare fermentation liquid culture medium: starch 10g, ferrous sulfate 0.01g, potassium nitrate 0.8g, dipotassium hydrogen phosphate 0.7g, magnesium sulfate 1g, tryptophan 0.5g add water to 1000ml, pH is 7.0. ...

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Abstract

The invention discloses a Duganella bacterium and its application. The Duganella bacterium is sp. B2 CGMCC No:2056. Culturing Duganella sp. B2 CGMCC No:2056 will get purple bacteriocin. The fermentingcondition for the bacterial strain is: culturing 24-60 h under 4-28 DEG C. The fermenting culture medium contains in each liter: starch 10-20 g, ferrisulphas 0.01-0.05 g, potassium nitrate 0.8-1.2 g,dipotassium hydrogen phosphate 0.5-1 g, and magnesium sulfate 0.5-1 g. The pH value of the fermenting culture medium is 6-9. The purple bacteriocin from the bacterial strain is a natural product, andfree from any hidden harm due to artificial pigment. Tests show that the purple bacteriocin is of good biologic activity, will generate no side effect on animal bodies, and can be widely used in suchfields as medicine and industries, etc.

Description

Technical field [0001] The invention relates to a strain of Dujuni and its application. Background technique [0002] Pigments are generally divided into synthetic pigments and natural pigments, both of which have been widely used in food colorants, daily cosmetics and feed additives. Artificially synthesized pigments refer to organic pigments prepared by chemical synthesis methods, which are mainly extracted from coal tar or synthesized using aromatic compounds such as benzene, toluene, naphthalene, and aniline as raw materials. After the emergence of artificial synthetic pigments, it was quickly accepted by users for its bright color, strong coloring power, good stability, convenient color matching, and low price. However, with the development of modern medicine, food hygiene and other disciplines, people found that synthetic Pigments have toxicological problems, and some are harmful to human health and even induce cancer. Therefore, more and more countries have stopped using...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04
Inventor 邢新会王海胜卢元薛园姜瑞波娄恺魏东
Owner TSINGHUA UNIV
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